Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis

Version 1 : Received: 22 April 2020 / Approved: 23 April 2020 / Online: 23 April 2020 (15:29:18 CEST)
Version 2 : Received: 18 April 2021 / Approved: 19 April 2021 / Online: 19 April 2021 (11:54:06 CEST)

How to cite: Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints 2020, 2020040419. https://doi.org/10.20944/preprints202004.0419.v1 Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints 2020, 2020040419. https://doi.org/10.20944/preprints202004.0419.v1

Abstract

Polymerase chain reactions are helpful diagnostic methods to accurately quantitate the intensities of infections of various soil-transmitted helminths, especially in the low-intensity infection samples. Method of DNA extraction hugely impacts the outcomes of the diagnostic method. Trichuris trichiura is one of the three major helminths prevalent world-wide and accurate estimation of their loads in stool is affected by the method of DNA extraction. We meta-analyze two studies by dividing them into all together 6 sub-studies. The objectives of the meta-analysis required the two studies to be divided into sub-studies as the different methods of DNA extractions could not be combined. We found that the inclusion of the bead-beating step during DNA extraction significantly increases the sensitivity of the test.

Keywords

Trichuris trichiura; polymerase chain reaction; bead-beating; DNA extraction; meta-analysis

Subject

Biology and Life Sciences, Immunology and Microbiology

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.