Version 1
: Received: 22 April 2020 / Approved: 23 April 2020 / Online: 23 April 2020 (15:29:18 CEST)
Version 2
: Received: 18 April 2021 / Approved: 19 April 2021 / Online: 19 April 2021 (11:54:06 CEST)
How to cite:
Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints2020, 2020040419. https://doi.org/10.20944/preprints202004.0419.v1
Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints 2020, 2020040419. https://doi.org/10.20944/preprints202004.0419.v1
Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints2020, 2020040419. https://doi.org/10.20944/preprints202004.0419.v1
APA Style
Rana, D., & Pokhrel, N. (2020). Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints. https://doi.org/10.20944/preprints202004.0419.v1
Chicago/Turabian Style
Rana, D. and Nischal Pokhrel. 2020 "Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis" Preprints. https://doi.org/10.20944/preprints202004.0419.v1
Abstract
Polymerase chain reactions are helpful diagnostic methods to accurately quantitate the intensities of infections of various soil-transmitted helminths, especially in the low-intensity infection samples. Method of DNA extraction hugely impacts the outcomes of the diagnostic method. Trichuris trichiura is one of the three major helminths prevalent world-wide and accurate estimation of their loads in stool is affected by the method of DNA extraction. We meta-analyze two studies by dividing them into all together 6 sub-studies. The objectives of the meta-analysis required the two studies to be divided into sub-studies as the different methods of DNA extractions could not be combined. We found that the inclusion of the bead-beating step during DNA extraction significantly increases the sensitivity of the test.
Keywords
Trichuris trichiura; polymerase chain reaction; bead-beating; DNA extraction; meta-analysis
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.