Preprint Review Version 1 This version is not peer-reviewed

Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis

Version 1 : Received: 22 April 2020 / Approved: 23 April 2020 / Online: 23 April 2020 (15:29:18 CEST)

How to cite: Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints 2020, 2020040419 (doi: 10.20944/preprints202004.0419.v1). Rana, D.; Pokhrel, N. Effect of Bead-Beating on DNA Extraction from Trichuris trichiura Positive Stool Samples: A Meta-Analysis. Preprints 2020, 2020040419 (doi: 10.20944/preprints202004.0419.v1).

Abstract

Polymerase chain reactions are helpful diagnostic methods to accurately quantitate the intensities of infections of various soil-transmitted helminths, especially in the low-intensity infection samples. Method of DNA extraction hugely impacts the outcomes of the diagnostic method. Trichuris trichiura is one of the three major helminths prevalent world-wide and accurate estimation of their loads in stool is affected by the method of DNA extraction. We meta-analyze two studies by dividing them into all together 6 sub-studies. The objectives of the meta-analysis required the two studies to be divided into sub-studies as the different methods of DNA extractions could not be combined. We found that the inclusion of the bead-beating step during DNA extraction significantly increases the sensitivity of the test.

Subject Areas

Trichuris trichiura; polymerase chain reaction; bead-beating; DNA extraction; meta-analysis

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