Version 1
: Received: 18 April 2020 / Approved: 19 April 2020 / Online: 19 April 2020 (02:18:01 CEST)
How to cite:
Han, H.; Yang, Y.; Liu, B.; Tian, J.; Zhu, W.; Wang, J.; Lei, H. Chalcomoracin Prevents Vitreous-Induced Activation of AKT and Migration of Retinal Pigment Epithelial Cells. Preprints2020, 2020040312 (doi: 10.20944/preprints202004.0312.v1).
Han, H.; Yang, Y.; Liu, B.; Tian, J.; Zhu, W.; Wang, J.; Lei, H. Chalcomoracin Prevents Vitreous-Induced Activation of AKT and Migration of Retinal Pigment Epithelial Cells. Preprints 2020, 2020040312 (doi: 10.20944/preprints202004.0312.v1).
Cite as:
Han, H.; Yang, Y.; Liu, B.; Tian, J.; Zhu, W.; Wang, J.; Lei, H. Chalcomoracin Prevents Vitreous-Induced Activation of AKT and Migration of Retinal Pigment Epithelial Cells. Preprints2020, 2020040312 (doi: 10.20944/preprints202004.0312.v1).
Han, H.; Yang, Y.; Liu, B.; Tian, J.; Zhu, W.; Wang, J.; Lei, H. Chalcomoracin Prevents Vitreous-Induced Activation of AKT and Migration of Retinal Pigment Epithelial Cells. Preprints 2020, 2020040312 (doi: 10.20944/preprints202004.0312.v1).
Abstract
Retinal pigment epithelial (RPE) cells are the major cell type in the epi- or sub-retinal membranes in the pathogenesis of proliferative vitreoretinopathy (PVR), which is a blinding fibrotic eye disease and still short of effective medicine. The purpose of this study is to demonstrate if Chalocomoracin (CMR), a novel purified compound from fungus-infected mulberry leaves, is able to inhibit vitreous-induced signaling events and cellular responses intrinsic to PVR. Our studies have revealed that the CMR IC50 for ARPE-19 cells is 35.5 μM at 72 hours, and that 5 μM CMR inhibits vitreous-induced Akt activation and p53 suppression; in addition we have discovered that this chemical effectively blocks vitreous-stimulated proliferation, migration and contraction of ARPE-19 cells, suggesting that CMR is a promising PVR prophylactic.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Haote Han