Article
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The Making of Transgenic Drosophila guttifera
Version 1
: Received: 7 April 2020 / Approved: 8 April 2020 / Online: 8 April 2020 (06:08:53 CEST)
A peer-reviewed article of this Preprint also exists.
Shittu, M.; Steenwinkel, T.; Koshikawa, S.; Werner, T. The Making of Transgenic Drosophila guttifera. Methods Protoc. 2020, 3, 31. Shittu, M.; Steenwinkel, T.; Koshikawa, S.; Werner, T. The Making of Transgenic Drosophila guttifera. Methods Protoc. 2020, 3, 31.
Abstract
The complex color patterns on the wings and body of Drosophila guttifera (D. guttifera) are emerging as model systems for studying evolutionary and developmental processes. Studies regarding these processes depend on overexpression and down-regulation of developmental genes, which ultimately rely upon an effective transgenic system. Methods describing transgenesis in Drosophila melanogaster (D. melanogaster) have been extensively reported in several studies. However, these methods cannot be directly applied to D. guttifera due to the low egg production rate and very delicate embryos’ pre-injection treatment requirements. In this protocol, we describe extensively a comprehensive method used for generating transgenic D. guttifera. Using the protocol described here, we are able to establish transgenic lines, identifiable by the expression of Enhanced Green Fluorescent Protein (EGFP) in the eye disks of D. guttifera larvae. The entire procedure, from injection to screening for transgenic larvae, can be completed in approximately 30 days and should be relatively easy to adapt to other non-model Drosophila species, for which no white-eyed mutants exist.
Keywords
microinjection; Drosophila guttifera; transgenesis, piggyBac
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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