Preprint Brief Report Version 3 This version is not peer-reviewed

A Flexible Genome-Scale SARS-CoV-2 Clone Resource

Version 1 : Received: 31 March 2020 / Approved: 2 April 2020 / Online: 2 April 2020 (04:15:54 CEST)
Version 2 : Received: 3 May 2020 / Approved: 4 May 2020 / Online: 4 May 2020 (18:28:01 CEST)
Version 3 : Received: 16 June 2020 / Approved: 16 June 2020 / Online: 16 June 2020 (09:37:50 CEST)
Version 4 : Received: 19 June 2020 / Approved: 21 June 2020 / Online: 21 June 2020 (15:38:18 CEST)
Version 5 : Received: 7 July 2020 / Approved: 7 July 2020 / Online: 7 July 2020 (18:09:21 CEST)

How to cite: Kim, D.; Knapp, J.J.; Kuang, D.; Chawla, A.; Cassonnet, P.; Lee, H.; Sheykhkarimli, D.; Samavarchi-Tehrani, P.; Abdouni, H.; Rayhan, A.; Pogoutse, O.; Coyaud, E.; van der Werf, S.; Demeret, C.; Gingras, A.; Taipale, M.; Raught, B.; Jacob, Y.; Roth, F.P. A Flexible Genome-Scale SARS-CoV-2 Clone Resource. Preprints 2020, 2020040009 (doi: 10.20944/preprints202004.0009.v3). Kim, D.; Knapp, J.J.; Kuang, D.; Chawla, A.; Cassonnet, P.; Lee, H.; Sheykhkarimli, D.; Samavarchi-Tehrani, P.; Abdouni, H.; Rayhan, A.; Pogoutse, O.; Coyaud, E.; van der Werf, S.; Demeret, C.; Gingras, A.; Taipale, M.; Raught, B.; Jacob, Y.; Roth, F.P. A Flexible Genome-Scale SARS-CoV-2 Clone Resource. Preprints 2020, 2020040009 (doi: 10.20944/preprints202004.0009.v3).

Abstract

The world is facing a major health crisis, the global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus, for which no approved antiviral agents or vaccines are currently available. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available via Addgene. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Subject Areas

SARS-CoV-2; coding sequence collection; gateway-compatible

Comments (1)

Comment 1
Received: 16 June 2020
Commenter: Da Kuang
Commenter's Conflict of Interests: Author
Comment: Manuscript updated to include new TEV and deletion variant clones.
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