Preprint Article Version 1 This version is not peer-reviewed

Knockout of MMP3 Weakened Solid Tumor Organoids and Cancer Extracellular Vesicles

Version 1 : Received: 25 March 2020 / Approved: 25 March 2020 / Online: 25 March 2020 (05:25:09 CET)

How to cite: Taha, E.A.; Sogawa, C.; Okusha, Y.; Kawai, H.; Oo, M.W.; El-Seoudi, A.; Lu, Y.; Nagatsuka, H.; Kubota, S.; Satoh, A.; Okamoto, K.; Eguchi, T. Knockout of MMP3 Weakened Solid Tumor Organoids and Cancer Extracellular Vesicles. Preprints 2020, 2020030371 (doi: 10.20944/preprints202003.0371.v1). Taha, E.A.; Sogawa, C.; Okusha, Y.; Kawai, H.; Oo, M.W.; El-Seoudi, A.; Lu, Y.; Nagatsuka, H.; Kubota, S.; Satoh, A.; Okamoto, K.; Eguchi, T. Knockout of MMP3 Weakened Solid Tumor Organoids and Cancer Extracellular Vesicles. Preprints 2020, 2020030371 (doi: 10.20944/preprints202003.0371.v1).

Abstract

The tumor organoid (tumoroid) model in three-dimensional (3D) culture systems has been developed to reflect more closely the in vivo tumors than 2D-cultured tumor cells. Notably, extracellular vesicles (EVs) are efficiently collectible from the culture supernatant of gel-free tumoroids. Matrix metalloproteinase (MMP) 3 is a multi-functional factor playing crucial roles in tumor progression. However, roles of MMP3 within tumor growth and EVs have not unveiled. Here, we investigated the protumorigenic roles of MMP3 on integrities of tumoroids and EVs. We generated MMP3-knockout (KO) cells using the CRISPR/Cas9 system from rapidly metastatic LuM1 tumor cells. Moreover, we established fluorescent cell lines with palmitoylation signal-fused fluorescent proteins (tdTomato and enhanced GFP). Then we confirmed the exchange of EVs between cellular populations and tumoroids. LuM1-tumoroids released large EVs (300-1000 nm) and small EVs (50-200 nm) while the knockout of MMP3 resulted in the additional release of broken EVs from tumoroids. The loss of MMP3 leads to a significant reduction in tumoroid size and to the development of the necrotic area within tumoroids. MMP3 and CD9 (a category-1 EV marker tetraspanin protein) were significantly down-regulated in MMP3-KO cells and their EV fraction. These weakened phenotypes by MMP3 KO were markedly rescued by the addition of MMP3-rich EVs or conditioned medium (CM) collected from LuM1-tumoroids, which caused a dramatic rise in the expression of MMP3, CD9, and Ki-67 (a marker of proliferating cells) in the MMP3-null/CD9-low tumoroids. Notably, MMP3 enriched in tumoroids-derived EVs and CM deeply penetrated into recipient MMP3-KO tumoroids, resulting in a remarkable enlargement of solid tumoroids, while MMP3-null EVs did not. These data demonstrate that EVs can mediate molecular transfer of MMP3 resulting in increasing the proliferation and CD9+ tumorigenesis, indicating crucial roles of MMP3 in tumor progression.

Subject Areas

matrix metalloproteinase 3 (MMP3); extracellular vesicles (EVs); tumoroid; tumor organoid; tumorigenesis; three-dimensional (3D) culture system

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