Adewumi, A.S.; Taah, K.J.; Asare, P.A.; Adu, M.O.; Agre, P.A. Assessment of Genetic Diversity of Dioscorea praehensilis (Berth.) Collected from Central Region, Ghana Using Simple Sequence Repeat (SSR) Markers. Preprints2020, 2020030137
APA Style
Adewumi, A.S., Taah, K.J., Asare, P.A., Adu, M.O., & Agre, P.A. (2020). Assessment of Genetic Diversity of <em>Dioscorea praehensilis</em> (Berth.) Collected from Central Region, Ghana Using Simple Sequence Repeat (SSR) Markers. Preprints. https://doi.org/
Chicago/Turabian Style
Adewumi, A.S., Michael O. Adu and Paterne A. Agre. 2020 "Assessment of Genetic Diversity of <em>Dioscorea praehensilis</em> (Berth.) Collected from Central Region, Ghana Using Simple Sequence Repeat (SSR) Markers" Preprints. https://doi.org/
Abstract
Dioscorea praehensilis Berth is one of the wild yam species resistance to many yam disease (yam anthracnose disease and yam mosaic virus) grow in Ghana especially in the cocoa grown regions of the country. It is a crucial crop that has been known to contribute to poverty reduction and food gap. Genetic diversity in this yam species has been discovered to be eroding and neglected. In this study we evaluated the genetic diversity among 43 D. praehensilis collected from Ghana using simple sequence repeat (SSR). Using 11 SSR marker, a total of 99 number of alleles were generated with an average of 8.48 alleles per locus. The mean gene diversity was 0.81, mean polymorphism information content was 0.82 while mean Shannon information index was 1.94. Principal coordinate analysis (PCoA) revealed a contribution of 40.16% of the first three coordinate axes and grouped the 43 morphotypes into 2 groups while hierarchical cluster through UPGMA revealed the presence of 3 main clusters. Molecular variance (AMOVA) alongside the Fst revealed low genetic diversity and differentiation among accessions and population. Result of this study assess the genetic diversity and will facilitate the use D. praehensilis as sources of resistance gene into yam breeding program.
Keywords
genetic diversity; Dioscorea praehensilis; SSR markers; Ghana
Subject
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
