Version 1
: Received: 29 February 2020 / Approved: 1 March 2020 / Online: 1 March 2020 (11:52:27 CET)
How to cite:
Pauk, M.; Amigo-Benavent, M.; Patel, B.; Jakeman, P.M.; Carson, B.P. Casein Protein Hydrolysate Fed Human Serum From Older Adults Regulates In Vitro Muscle Protein Synthesis, Muscle Protein Breakdown and Myotube Growth. Preprints2020, 2020030010 (doi: 10.20944/preprints202003.0010.v1).
Pauk, M.; Amigo-Benavent, M.; Patel, B.; Jakeman, P.M.; Carson, B.P. Casein Protein Hydrolysate Fed Human Serum From Older Adults Regulates In Vitro Muscle Protein Synthesis, Muscle Protein Breakdown and Myotube Growth. Preprints 2020, 2020030010 (doi: 10.20944/preprints202003.0010.v1).
Cite as:
Pauk, M.; Amigo-Benavent, M.; Patel, B.; Jakeman, P.M.; Carson, B.P. Casein Protein Hydrolysate Fed Human Serum From Older Adults Regulates In Vitro Muscle Protein Synthesis, Muscle Protein Breakdown and Myotube Growth. Preprints2020, 2020030010 (doi: 10.20944/preprints202003.0010.v1).
Pauk, M.; Amigo-Benavent, M.; Patel, B.; Jakeman, P.M.; Carson, B.P. Casein Protein Hydrolysate Fed Human Serum From Older Adults Regulates In Vitro Muscle Protein Synthesis, Muscle Protein Breakdown and Myotube Growth. Preprints 2020, 2020030010 (doi: 10.20944/preprints202003.0010.v1).
Abstract
In this study we used a recently developed ex vivo-in vitro model to assess the effect of feeding older adults a casein protein hydrolysate (CPH) compared with non-bioactive non-essential amino acid (NEAA) supplement on Muscle Protein Synthesis (MPS) and Breakdown (MPB). Serum from six healthy older males following overnight fast and 60 min postprandial ingestion of CPH or NEAA (0.33 g.kg-1 body mass) was used to condition C2C12 myotube media. CPH-fed serum significantly increased MPS compared to fasted serum. In addition, CPH-fed serum induced myotube growth and markedly suppressed atrogin-1, but not MuRF1, expression. Comparatively, no change in MPS, myotube growth and gene expression was observed following NEAA-fed serum treatment. CPH-fed serum from older adults stimulated de novo MPS, suppressed markers of protein breakdown and resulted in myotube growth, indicating a potential role for CPH as a dietary protein source to prevent age-related sarcopenia.
Subject Areas
skeletal muscle; muscle protein synthesis; muscle protein breakdown; serum; hydrolysate
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Martina Pauk
Philip M. Jakeman
