Working Paper Article Version 1 This version is not peer-reviewed

Characterization of Essential Fatty Acid Semicarbazide (EFASC) from Linum usitatissium as a Safe and Effective Adjuvant

Version 1 : Received: 17 February 2020 / Approved: 18 February 2020 / Online: 18 February 2020 (11:15:32 CET)

How to cite: Darweesh, M.; Hady, A.; salman, H. Characterization of Essential Fatty Acid Semicarbazide (EFASC) from Linum usitatissium as a Safe and Effective Adjuvant. Preprints 2020, 2020020267 Darweesh, M.; Hady, A.; salman, H. Characterization of Essential Fatty Acid Semicarbazide (EFASC) from Linum usitatissium as a Safe and Effective Adjuvant. Preprints 2020, 2020020267

Abstract

Bacterial infections are world-wide problem and resistant bacteria is increasing globally that lead to treatments failure. Thus, it is important to find new techniques that are effective for the treatment antibiotic-resistant microorganisms. Green medicine used for the medication of different bacterial infection. Therefore , the study aimed to evaluate the immunomodulatory activity of essential fatty acid semi carbazide (EFASC) compounds extracted from flaxseed to eradicate resistance pathogens . Crud extracts of Linum usitatissimum was extracted by hexane for extraction of EFASC. The results of LD50 appear that a live suspension of E.coli was 0.49×107 ,while HK E.coli was 108 , 125 mg /ml were the optimum dose in stimulate immune response of EFASC which gave maximum dose of total WBC , Neutrophil and Monocyte count. The immunomodulatory activity appear that E.coli + EFASC released moderate levels of IL-4 . HK E.coli release higher IL-4 lead to increase the hypersensitivity and hyper responses of immune system. HK + EFASC immunogen revealed moderate increased in IL-12 mean level 0.99 pg /ml, HK bacteria recorded 1.49 pg/ml, while the mean of EFASC revealed no significant different compare with control. An effective protection was observed in immunized groups with HK E.coli and EFASC challenged with 100 of LD50 of a live E.coli.

Supplementary and Associated Material

Subject Areas

essential fatty acid semi carbazide; ID50; IL-4; IL-12

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.