Preprint Article Version 1 This version is not peer-reviewed

Drug and Vaccine Design against Novel Coronavirus (2019-nCoV) Spike Protein through Computational Approach

Version 1 : Received: 4 February 2020 / Approved: 5 February 2020 / Online: 5 February 2020 (15:34:15 CET)

How to cite: Kumar, S. Drug and Vaccine Design against Novel Coronavirus (2019-nCoV) Spike Protein through Computational Approach. Preprints 2020, 2020020071 (doi: 10.20944/preprints202002.0071.v1). Kumar, S. Drug and Vaccine Design against Novel Coronavirus (2019-nCoV) Spike Protein through Computational Approach. Preprints 2020, 2020020071 (doi: 10.20944/preprints202002.0071.v1).

Abstract

The recent outbreak of the new virus in Wuhan city, China from the sea food market has led to the identification of a new strain called the corona virus and named as novel corona virus (2019-nCoV) belonging to Coronaviridae family. This has created major havoc and concern due to the mortality of 250 persons and affecting more than 10,000 people. This virus causes sudden fever, pneumonia and also kidney failure. In this study a computational approach is proposed for drug and vaccine design. The spike protein sequences were collected from a protein database and analysed with various bioinformatics tools to identify suitable natural inhibitors for the N-terminal receptor binding domain of spike protein. Also, it is attempted to identify suitable vaccine candidates by identifying B-Cell and T-cell epitopes. In the drug design, the tanshinone Iia and methyl Tanshinonate were identified as natural inhibitors based on the docking score. In the vaccine design, B-cell epitope VLLPLVSSQCVNLTTRTQLPPAYTN was found to have the highest antigenicity. FVFLVLLPL of MHC class-I allele and FVFLVLLPL of MHC class-II allele were identified as best peptides based on a number of alleles and antigencity scores. The present study identifies natural inhibitors and putative antigenic epitopes which may be useful as effective drug and vaccine candidates for the eradication of novel corona virus.

Subject Areas

2019-nCoV; novel corona virus; Wuhan virus; drug; vaccine; spike protein; epitope; vaccine design

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