Version 1
: Received: 13 December 2019 / Approved: 16 December 2019 / Online: 16 December 2019 (11:29:09 CET)
How to cite:
Fuster, B.; Tormo, N.; Salvador, C.; García, N.; González-Candelas, F.; Gimeno, C. Genetic features of Multi Drug Resistant (MDR) Klebsiella pneumoniae and its Plasmids. Preprints2019, 2019120215. https://doi.org/10.20944/preprints201912.0215.v1
Fuster, B.; Tormo, N.; Salvador, C.; García, N.; González-Candelas, F.; Gimeno, C. Genetic features of Multi Drug Resistant (MDR) Klebsiella pneumoniae and its Plasmids. Preprints 2019, 2019120215. https://doi.org/10.20944/preprints201912.0215.v1
Fuster, B.; Tormo, N.; Salvador, C.; García, N.; González-Candelas, F.; Gimeno, C. Genetic features of Multi Drug Resistant (MDR) Klebsiella pneumoniae and its Plasmids. Preprints2019, 2019120215. https://doi.org/10.20944/preprints201912.0215.v1
APA Style
Fuster, B., Tormo, N., Salvador, C., García, N., González-Candelas, F., & Gimeno, C. (2019). Genetic features of Multi Drug Resistant (MDR) Klebsiella pneumoniae and its Plasmids. Preprints. https://doi.org/10.20944/preprints201912.0215.v1
Chicago/Turabian Style
Fuster, B., Fernando González-Candelas and Concepcion Gimeno. 2019 "Genetic features of Multi Drug Resistant (MDR) Klebsiella pneumoniae and its Plasmids" Preprints. https://doi.org/10.20944/preprints201912.0215.v1
Abstract
Klebsiella pneumoniae, a major cause of both hospital and community-acquired infections, is listed by the World Health Organization as a critical priority antibiotic- resistant bacterial pathogen. With the appearance of sequencing techniques such as Next-generation Sequencing (NGS), there is the possibility to obtain the whole genome of the bacteria, getting to know all antimicrobial resistance determinants. The purpose of this study has been to apply this new technology to clinical microbiology, in order to characterize the resistome present in carbapenem-resistant K.pneumoniae strains isolated in a tertiary hospital in Valencia, Spain. A total of 234 isolates were prepared for whole-genome sequencing with Ilumina MiSeq, and sequences were later studied for antimicrobial resistance genes, sequence-typing and plasmids. Sequence-typing showed four major circulating clones in our hospital settings: ST11, ST307, ST101 and ST147, carrying different plasmids and different resistance determinants such as OXA-48 and NDM-1 carbapenemase. Application of new technologies such as whole-genome sequencing in clinical microbiology gives advantages when it comes to rapid therapy adjustment, consequently improving the patient’s clinical outcomes.
Keywords
antimicrobial; resistance; plasmids; sequencing
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
