PreprintDatasetVersion 1Preserved in Portico This version is not peer-reviewed
Differential Gene Expression Associated with BMI, Gender, and IBS-subtype in Human White Blood Cells: Results from a Custom 250-plex Nanostring Probe Panel
Version 1
: Received: 12 December 2019 / Approved: 13 December 2019 / Online: 13 December 2019 (10:41:56 CET)
How to cite:
Robinson, J. Differential Gene Expression Associated with BMI, Gender, and IBS-subtype in Human White Blood Cells: Results from a Custom 250-plex Nanostring Probe Panel. Preprints2019, 2019120180. https://doi.org/10.20944/preprints201912.0180.v1
Robinson, J. Differential Gene Expression Associated with BMI, Gender, and IBS-subtype in Human White Blood Cells: Results from a Custom 250-plex Nanostring Probe Panel. Preprints 2019, 2019120180. https://doi.org/10.20944/preprints201912.0180.v1
Robinson, J. Differential Gene Expression Associated with BMI, Gender, and IBS-subtype in Human White Blood Cells: Results from a Custom 250-plex Nanostring Probe Panel. Preprints2019, 2019120180. https://doi.org/10.20944/preprints201912.0180.v1
APA Style
Robinson, J. (2019). Differential Gene Expression Associated with BMI, Gender, and IBS-subtype in Human White Blood Cells: Results from a Custom 250-plex Nanostring Probe Panel. Preprints. https://doi.org/10.20944/preprints201912.0180.v1
Chicago/Turabian Style
Robinson, J. 2019 "Differential Gene Expression Associated with BMI, Gender, and IBS-subtype in Human White Blood Cells: Results from a Custom 250-plex Nanostring Probe Panel" Preprints. https://doi.org/10.20944/preprints201912.0180.v1
Abstract
Chronic gastro-abdominal pain with altered bowel habits are associated with pathologies including gastroenteritis, autoimmune and inflammatory bowel disease, and irritable bowel syndrome (IBS). In IBS, diagnostic evidence of infection or inflammation are absent, yet symptoms nonetheless include chronic abdominal pain and alterations of stool frequency and consistency, with most common subtypes including diarrhea-predominant (IBS-D) or constipation predominant (IBS-C). [1, 2] IBS is a common clinical complaint in westernized nations, with females more frequently diagnosed than males [3]. Obesity is also associated with increased likelihood of chronic pain [4], and is associated with intestinal dysbiosis, and systemic inflammatory signatures [5]. Improving personalized medicine therefore requires patient stratification based on a combination of biological factors contributing to the individual’s symptom and biomarker spectrum. The “buffy coat” method isolates leukocytes and PBMCs from whole blood, comprises the immune cell population in peripheral blood. These cells are relatively inactive but are primed to respond to generalized and localized immune activation signals; they are under active investigation as diagnostic biomarkers [6]. Collection of buffy coat is a standard method in clinical laboratories; detection of immune activation via RNA expression biomarkers will be informative of differential biological response, and be tractable to collect in clinical settings. Results of differential expression, pathway analysis, and cell-type abundance analysis from buffy coat RNA, is presented here using variables for BMI, Gender, and IBS-subtype from publicly available Nanostring RNA expression and phenotypic data from the NCBI GEO database.
Keywords
IBS; BMI; Nanostring; White Blood Cells; RNA expression
Subject
Medicine and Pharmacology, Pathology and Pathobiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
