Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

Dhanak Gupta
,
Melissa Silva
,
Karolina Radziun
,
Diana Martinez
ORCID logo ,
Christopher Hill
,
Julie Marshall
,
Vanessa Hearnden
ORCID logo ,
Miguel Puertas-Mejia
,
Gwendolen Reilly
* ORCID logo
Version 1 : Received: 4 December 2019 / Approved: 9 December 2019 / Online: 9 December 2019 (03:45:59 CET)

A peer-reviewed article of this Preprint also exists.

Gupta, D.; Silva, M.; Radziun, K.; Martinez, D.C.; Hill, C.J.; Marshall, J.; Hearnden, V.; Puertas-Mejia, M.A.; Reilly, G.C. Fucoidan Inhibition of Osteosarcoma Cells Is Species and Molecular Weight Dependent. Mar. Drugs 2020, 18, 104. Gupta, D.; Silva, M.; Radziun, K.; Martinez, D.C.; Hill, C.J.; Marshall, J.; Hearnden, V.; Puertas-Mejia, M.A.; Reilly, G.C. Fucoidan Inhibition of Osteosarcoma Cells Is Species and Molecular Weight Dependent. Mar. Drugs 2020, 18, 104.

Abstract

Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anticancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10-50kDa), medium (MMW, 50-100kDa) and high (HMW, >100kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had highest the levels of sugars, acids and sulphation among molecular weight fractions. There was a dose dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in sub G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress induced apoptosis-like cell death for F. vesiculosus fucoidan but features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.

Keywords

apoptosis; necrosis; brown algae,; mitochondria; mg63 cells; fucoidan; molecular weight fraction; crude extract; cell cycle; transmission electron microscopy

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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