Version 1
: Received: 14 November 2019 / Approved: 15 November 2019 / Online: 15 November 2019 (08:48:50 CET)
How to cite:
Utispan, K.; Koontongkaew, S. Macrophage Migration Inhibitory Factor Modulated Proliferation, Cell Cycle, and Apoptotic Activity in Head and Neck Cancer Cell Lines. Preprints2019, 2019110177. https://doi.org/10.20944/preprints201911.0177.v1
Utispan, K.; Koontongkaew, S. Macrophage Migration Inhibitory Factor Modulated Proliferation, Cell Cycle, and Apoptotic Activity in Head and Neck Cancer Cell Lines. Preprints 2019, 2019110177. https://doi.org/10.20944/preprints201911.0177.v1
Utispan, K.; Koontongkaew, S. Macrophage Migration Inhibitory Factor Modulated Proliferation, Cell Cycle, and Apoptotic Activity in Head and Neck Cancer Cell Lines. Preprints2019, 2019110177. https://doi.org/10.20944/preprints201911.0177.v1
APA Style
Utispan, K., & Koontongkaew, S. (2019). Macrophage Migration Inhibitory Factor Modulated Proliferation, Cell Cycle, and Apoptotic Activity in Head and Neck Cancer Cell Lines. Preprints. https://doi.org/10.20944/preprints201911.0177.v1
Chicago/Turabian Style
Utispan, K. and Sittichai Koontongkaew. 2019 "Macrophage Migration Inhibitory Factor Modulated Proliferation, Cell Cycle, and Apoptotic Activity in Head and Neck Cancer Cell Lines" Preprints. https://doi.org/10.20944/preprints201911.0177.v1
Abstract
Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine that contributes to the progression of several cancers. MIF overexpression has been reported in head and neck squamous cell carcinoma (HNSCC) patients. However, the exact role of MIF in HNSCC is not fully understood. Our aim was to evaluate the amount of secreted MIF and the role of MIF in the proliferation, cell cycle, and apoptosis in HNSCC cell lines. The MIF levels in conditioned media from human primary (HN18 and HN30) and metastatic (HN17 and HN31) HNSCC cell lines were evaluated using ELISA. The HNSCC cell lines were treated with recombinant MIF and its effect on proliferation, cell cycle, and apoptotic status was determined by MTT and flow cytometry, respectively. The HNSCC-secreted MIF concentration ranged from 49.33‒860 pg/ml. Exogenous MIF (25 ng/ml) significantly increased HN18, HN30, and HN31 cell proliferation. Moreover, MIF induced cell cycle progression and inhibited apoptosis in these cells. However, MIF did not affect growth or apoptosis in HN17 cell. In conclusion, the HNSCC cell lines were evaluated secrete MIF. Exogenous MIF promotes various effects on proliferation, cell cycle, and apoptosis in HNSCC cells.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
