The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research

How to cite: so, S.; Lee, Y.; Park, J.; Lee, J.-Y.; Kim, D.; Hwang, J.; Shin, J.; Choi, J.; Han, Y.; Kang, S.; Dutton, J. R.; Seo, E.-J.; Lee, B. H.; Kim, C. J.; Mitalipov, S.; Oh, S.; Kang, E. The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research. Preprints 2019, 2019110094. https://doi.org/10.20944/preprints201911.0094.v1 so, S.; Lee, Y.; Park, J.; Lee, J.-Y.; Kim, D.; Hwang, J.; Shin, J.; Choi, J.; Han, Y.; Kang, S.; Dutton, J. R.; Seo, E.-J.; Lee, B. H.; Kim, C. J.; Mitalipov, S.; Oh, S.; Kang, E. The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research. Preprints 2019, 2019110094. https://doi.org/10.20944/preprints201911.0094.v1

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MDPI and ACS Style

so, S.; Lee, Y.; Park, J.; Lee, J.-Y.; Kim, D.; Hwang, J.; Shin, J.; Choi, J.; Han, Y.; Kang, S.; Dutton, J. R.; Seo, E.-J.; Lee, B. H.; Kim, C. J.; Mitalipov, S.; Oh, S.; Kang, E. The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research. Preprints 2019, 2019110094. https://doi.org/10.20944/preprints201911.0094.v1

APA Style

so, S., Lee, Y., Park, J., Lee, J. Y., Kim, D., Hwang, J., Shin, J., Choi, J., Han, Y., Kang, S., Dutton, J. R., Seo, E. J., Lee, B. H., Kim, C. J., Mitalipov, S., Oh, S., & Kang, E. (2019). The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research. Preprints. https://doi.org/10.20944/preprints201911.0094.v1

Chicago/Turabian Style

so, S., Soojin Oh and Eunju Kang. 2019 "The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research" Preprints. https://doi.org/10.20944/preprints201911.0094.v1

Abstract

Poor survival of human pluripotent stem cells (hPSCs) following freezing, thawing, or passaging hinders maintenance and differentiation in stem cell research. Rho-associated kinases (ROCKs) play a crucial role in hPSC survival. To date, a typical ROCK inhibitor, Y-27632, has been the primary agent used in hPSC research. Here, we report that another ROCK inhibitor, fasudil, can be used as an alternative. Fasudil increased hPSC growth due to survival rather than proliferation following thawing and passaging, similar to Y-27632. It did not affect pluripotency and genetic integrity including mitochondrial genome (mtDNA). Notably, the genes related to metabolism, mTORC1, and TP53 have mainly displayed a faster recovery pattern with ROCK inhibitors than control. Furthermore, fasudil was confirmed as useful for the single dissociation of hPSCs and for aggregation. It also increased retinal pigment epithelium (RPE) differentiation and the survival of neural crest cells during differentiation. These findings suggest that fasudil can replace Y-27632 for use in stem cell research.

Keywords

hPSCs; ROCK inhibitors; fasudil; cell survival; metabolism; differentiation

Subject

Biology and Life Sciences, Cell and Developmental Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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