Preprint Article Version 1 This version is not peer-reviewed

Activity and Stability of Urease Enzyme Immobilized on Amberlite Resin

Version 1 : Received: 30 October 2019 / Approved: 30 October 2019 / Online: 30 October 2019 (04:59:29 CET)

How to cite: Al-Shams, J.K.J.; Hussein, M.A.; Al-Hakeim, H. Activity and Stability of Urease Enzyme Immobilized on Amberlite Resin. Preprints 2019, 2019100348 (doi: 10.20944/preprints201910.0348.v1). Al-Shams, J.K.J.; Hussein, M.A.; Al-Hakeim, H. Activity and Stability of Urease Enzyme Immobilized on Amberlite Resin. Preprints 2019, 2019100348 (doi: 10.20944/preprints201910.0348.v1).

Abstract

Immobilization of enzymes is a good field of study to expand the life of enzyme in and lowering the cost of the chemical processes such as separation processes. Urease is an important enzyme with medical and industrial applications. The aim of the present study is to prepare an immobilized urease on a strong cation exchange resin (Amberlite IR120 -Na) and study of its activity and stability. We monitored the liberation of Na ions in the collected fractions and searching for protein in the fractions as an indicators of immobilization by ion exchange phenomenon. Sodium is measured using atomic absorption spectroscopy techniques, while protein tested by Bradford’s method. Immobilized urease activity was evaluated by salicylate-hypochlorite method. The results indicated a complete immobilization of urease enzyme on the resin surface with reserving 92% of the activity of free enzyme. The immobilized urease enzyme on resin showed good stability and it has a 62% of its activity after 154 days of storage at room temperature. It is concluded that a new immobilized urease enzyme system is prepared with good enzyme activity and stability.

Subject Areas

Amberlite IR120 Na resin; urease; enzyme; immobilization

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