Preprint Article Version 1 This version is not peer-reviewed

Enterovirus 71 Represses Interleukin Enhancer Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects

Version 1 : Received: 14 October 2019 / Approved: 15 October 2019 / Online: 15 October 2019 (05:37:34 CEST)

How to cite: Jin, J.; Wang, W.; Ai, S.; Zhang, Q.; Wu, K.; Liu, Y.; Wu, J. Enterovirus 71 Represses Interleukin Enhancer Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects. Preprints 2019, 2019100161 (doi: 10.20944/preprints201910.0161.v1). Jin, J.; Wang, W.; Ai, S.; Zhang, Q.; Wu, K.; Liu, Y.; Wu, J. Enterovirus 71 Represses Interleukin Enhancer Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects. Preprints 2019, 2019100161 (doi: 10.20944/preprints201910.0161.v1).

Abstract

Enterovirus 71 (EV71) infection hand-foot-mouth disease (HFMD), meningoencephalitis, neonatal sepsis, and even fatal encephalitis in children, thereby representing a serious public health hazard. It is important to determine the mechanisms underlying the regulation of EV71 infection. In this study, we initially reveal that the interleukin enhancer binding factor 2 (ILF2) down-regulates EV71 50% tissue culture infective dose (TCID50), attenuates EV71 plaque formation unit (PFU), thereby repressing EV71 infection. Moreover, we reveal a distinct mechanism by which EV71 antagonizes ILF2-mediated antiviral effects. Chip data analyses show that ILF2 mRNA is reduced upon EV71 infection. Cellular studies indicate that EV71 infection represses ILF2 mRNA expression and protein production in human leukemic monocytes (THP-1) differentiated macrophages and in human rhabdomyosarcoma (RD) cells. Additionally, EV71 non-structural protein 2B interacts with ILF2 in human embryonic kidney (HEK293T) cells. Interestingly, in the presence of EV71 2B, ILF2 is translocated from the nucleus to the cytoplasm and co-localizes with 2B in the cytoplasm. Therefore, we reveal a distinct mechanism by which EV71 antagonizes ILF2-mediated antiviral effects by inhibiting ILF2 expression and promoting ILF2 translocation from the nucleus to cytoplasm through its 2B protein.

Subject Areas

Enterovirus 71; EV71; EV71 non-structural protein 2B; interleukin enhancer binding factor 2; ILF2; virus infection; virus replication

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