Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Prevalent pH Controls the Capacity of Galdieria maxima to Use Ammonia and Nitrate as a Nitrogen Source

Manuela Iovinella
ORCID logo ,
Dora Allegra Carbone
,
Diana Cioppa
,
Seth J Davis
ORCID logo ,
Michele Innangi
ORCID logo ,
Sabrina Esposito
ORCID logo ,
Claudia Ciniglia
* ORCID logo
Version 1 : Received: 7 October 2019 / Approved: 8 October 2019 / Online: 8 October 2019 (11:32:24 CEST)

A peer-reviewed article of this Preprint also exists.

Iovinella, M.; Carbone, D.A.; Cioppa, D.; Davis, S.J.; Innangi, M.; Esposito, S.; Ciniglia, C. Prevalent pH Controls the Capacity of Galdieria maxima to Use Ammonia and Nitrate as a Nitrogen Source. Plants 2020, 9, 232. Iovinella, M.; Carbone, D.A.; Cioppa, D.; Davis, S.J.; Innangi, M.; Esposito, S.; Ciniglia, C. Prevalent pH Controls the Capacity of Galdieria maxima to Use Ammonia and Nitrate as a Nitrogen Source. Plants 2020, 9, 232.

Abstract

Galdieria maxima is a polyextremophilic alga capable of diverse metabolic processes. Ammonia is widely used in culture media typical of laboratory growth. Recent reports that this species can grow on wastes promote the concept that G. maxima might have biotechnological utility. Accordingly, there is a need to know the range of pH levels that can support G. maxima growth in a given nitrogen source. Here, we examined the combined effect of pH and nitrate/ammonium source on the growth and long-term response of the photochemical process to a pH gradient in different G. maxima strains. All were able to use differing nitrogen sources, despite both the growth rate and photochemical activity were significantly affected by the combination with the pH. All strains acidified the NH4+-medium (pH<3); only G. maxima IPPAS P507 showed reduced capacity in lowering the pH from 6.5. pH was a limiting factor in nitrate uptake at pH≥6.5; noteworthy, at pH 5 on nitrate G. maxima ACUF551 showed a good growth performance, despite the alkalization of the medium.

Keywords

galdieria; pH; ammonium; nitrate

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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