Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium Sidoides DC Root Extract

Aistė Jekabsone; Inga Sile; Andrea Cochis; Marina Makrecka-Kuka; Goda Laucaityte; Elina Makarova; Lia Rimondini; Rasa Bernotiene; Lina Raudone; Evelina Vedlugaite; Rasa Baniene; Alina Smalinskiene; Nijole Savickiene; Maija Dambrova

doi:10.20944/preprints201909.0197.v1

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preprints.org > biology and life sciences > immunology and microbiology > doi: 10.20944/preprints201909.0197.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium Sidoides DC Root Extract

^* ,

Version 1 : Received: 16 September 2019 / Approved: 18 September 2019 / Online: 18 September 2019 (04:07:50 CEST)

A peer-reviewed article of this Preprint also exists.

Jekabsone, A.; Sile, I.; Cochis, A.; Makrecka-Kuka, M.; Laucaityte, G.; Makarova, E.; Rimondini, L.; Bernotiene, R.; Raudone, L.; Vedlugaite, E.; Baniene, R.; Smalinskiene, A.; Savickiene, N.; Dambrova, M. Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium sidoides DC Root Extract. Nutrients 2019, 11, 2829. Jekabsone, A.; Sile, I.; Cochis, A.; Makrecka-Kuka, M.; Laucaityte, G.; Makarova, E.; Rimondini, L.; Bernotiene, R.; Raudone, L.; Vedlugaite, E.; Baniene, R.; Smalinskiene, A.; Savickiene, N.; Dambrova, M. Investigation of Antibacterial and Antiinflammatory Activities of Proanthocyanidins from Pelargonium sidoides DC Root Extract. Nutrients 2019, 11, 2829.

Abstract

The study explores antibacterial, antiinflammatory and cytoprotective capacity of Pelargonium sidoides DC root extract (PSRE) and proanthocyanidin fraction from PSRE (PACN) under conditions characteristic for periodontal disease. Following previous finding that PACN exerts stronger suppression of Porphyromonas gingivalis compared to the effect on commensal Streptococcus salivarius, the current work continues antibacterial investigation on Staphylococcus aureus, Staphylococcus epidermidis, Aggregatibacter actinomycetemcomitans and Escherichia coli. PSRE and PACN are also studied for their ability to prevent gingival fibroblast cell death in the presence of bacteria or bacterial lipopolysaccharide (LPS), to block LPS- or LPS+IFNγ-induced release of inflammatory mediators, gene expression and surface antigen presentation. Both PSRE and PACN were more efficient in suppressing Staphylococcus and Aggregatibacter compared to Escherichia, prevented A. actinomycetemcomitans- and LPS induced death of fibroblasts, decreased LPS-induced release of interleukin 8 and prostaglandin E2 from fibroblasts and IL-6 from leukocytes, blocked expression of IL-1β, iNOS, and surface presentation of CD80 and CD86 in LPS+IFNγ-treated macrophages, and IL-1β and COX-2 expression in LPS-treated leukocytes. None of the investigated substances affected either the level of secretion or expression of TNFα. In conclusion, PSRE, and especially PACN, possess strong antibacterial, antiinflammatory and gingival tissue protecting properties under periodontitis mimicking conditions and are suggestable candidates for treatment of the disease.

Keywords

periodontitis; Pelargonium sidoides DC root extract; proanthocyanidins; bacteriotoxicity; inflammatory cytokines; gene expression; fibroblasts; macrophages; leukocytes

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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