Preprint Article Version 1 This version is not peer-reviewed

Comparative Extraction Efficacy of Different Organic Solvents for Leaf Chlorophylls and Carotenoids in (Portulaca oleracea L.) Varying with Growth Behavior and Stress Type

Version 1 : Received: 14 September 2019 / Approved: 15 September 2019 / Online: 15 September 2019 (15:04:55 CEST)

How to cite: Ahmadi, K.; Omidi, H.; Mehrnia, M.; Ur Rehman, H. Comparative Extraction Efficacy of Different Organic Solvents for Leaf Chlorophylls and Carotenoids in (Portulaca oleracea L.) Varying with Growth Behavior and Stress Type. Preprints 2019, 2019090152 (doi: 10.20944/preprints201909.0152.v1). Ahmadi, K.; Omidi, H.; Mehrnia, M.; Ur Rehman, H. Comparative Extraction Efficacy of Different Organic Solvents for Leaf Chlorophylls and Carotenoids in (Portulaca oleracea L.) Varying with Growth Behavior and Stress Type. Preprints 2019, 2019090152 (doi: 10.20944/preprints201909.0152.v1).

Abstract

The chlorophyll is one of the most important natural pigments used extensively in the food industry. Two important factors for the production of chlorophyll are the use of plants rich in chlorophyll and efficiency of extraction method. Present investigation was performed to compare the extraction of photosynthetic pigments by using solvents of different chemical nature. The purslane plants with different growth behavior viz. Scrollable and standing were grown under shade and sunshine stress condition. Different solvents including diethyl ether, 5% ethanol, pure acetone, 20% acetone, pure methanol and 10% methanol were used to extract chlorophyll and carotenoids from the purslane plant. The results indicated that stress, growth type and different solvents had a significant effect on the extraction of chlorophyll and carotenoids. Different trend was observed in extraction rate for chlorophylls and carotenoids. Among the solvents, pure methanol was the best for extraction of chl a. Methanol and acetone were appropriate solvents to achieve the highest amount of chlorophyll from plant tissues. Among different solvents, pure methanol for chl a, pure acetone and methanol for carotenoids were best solvent for purslane plant with a growing type scrollable of under shade.

Subject Areas

chlorophyll; carotenoids; methanol; photo-protection; phytoextraction; Portulaca oleracea

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