Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy

Kalina L. Tosheva; Yue Yuan; Pedro M. Pereira; Siân Culley; Ricardo Henriques

doi:10.20944/preprints201908.0319.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Version 1 : Received: 29 August 2019 / Approved: 30 August 2019 / Online: 30 August 2019 (08:22:58 CEST)

Super-Resolution Microscopy enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we overview new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.

Phototoxicity, Photodamage, Super-Resolution Microscopy, Fluorescence

Biology and Life Sciences, Other

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Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy

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