Preprint Article Version 1 This version is not peer-reviewed

Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy

Version 1 : Received: 29 August 2019 / Approved: 30 August 2019 / Online: 30 August 2019 (08:22:58 CEST)

How to cite: Tosheva, K.L.; Yuan, Y.; Pereira, P.M.; Culley, S.; Henriques, R. Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy. Preprints 2019, 2019080319 (doi: 10.20944/preprints201908.0319.v1). Tosheva, K.L.; Yuan, Y.; Pereira, P.M.; Culley, S.; Henriques, R. Between Life and Death: Reducing Phototoxicity in Super-Resolution Microscopy. Preprints 2019, 2019080319 (doi: 10.20944/preprints201908.0319.v1).

Abstract

Super-Resolution Microscopy enables non-invasive, molecule-specific imaging of the internal structure and dynamics of cells with sub-diffraction limit spatial resolution. One of its major limitations is the requirement for high-intensity illumination, generating considerable cellular phototoxicity. This factor considerably limits the capacity for live-cell observations, particularly for extended periods of time. Here, we overview new developments in hardware, software and probe chemistry aiming to reduce phototoxicity. Additionally, we discuss how the choice of biological model and sample environment impacts the capacity for live-cell observations.

Subject Areas

Phototoxicity, Photodamage, Super-Resolution Microscopy, Fluorescence

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