Preprint Brief Report Version 1 Preserved in Portico This version is not peer-reviewed

Molecular Identification of a Tentatively Novel Hantavirus in Malaysian Bronze Tube-Nosed Bat (Murina aenea)

Brigitta Zana
,
Gabor Kemenesi
* ORCID logo ,
Dora Buzas
,
Gabor Csorba
,
Tamas Gorfol
,
Faisal Ali Anwarali Khan
,
Nurul Farah Diyana Ahmad Tahir
,
Safia Zeghbib
,
Monika Madai
,
Henrietta Papp
,
Fanni Foldes
,
Peter Urban
,
Robert Herczeg
,
Gabor Endre Toth
,
Ferenc Jakab
*
Version 1 : Received: 31 July 2019 / Approved: 2 August 2019 / Online: 2 August 2019 (09:13:15 CEST)

A peer-reviewed article of this Preprint also exists.

Zana, B.; Kemenesi, G.; Buzás, D.; Csorba, G.; Görföl, T.; Khan, F.A.A.; Tahir, N.F.D.A.; Zeghbib, S.; Madai, M.; Papp, H.; Földes, F.; Urbán, P.; Herczeg, R.; Tóth, G.E.; Jakab, F. Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea). Viruses 2019, 11, 887. Zana, B.; Kemenesi, G.; Buzás, D.; Csorba, G.; Görföl, T.; Khan, F.A.A.; Tahir, N.F.D.A.; Zeghbib, S.; Madai, M.; Papp, H.; Földes, F.; Urbán, P.; Herczeg, R.; Tóth, G.E.; Jakab, F. Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea). Viruses 2019, 11, 887.

Abstract

In the past ten years several novel hantaviruses were discovered in shrews, moles and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the co-evolutionary analyses of most recent studies have raised the possibility of non-rodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia, 2015. Hantavirus specific nested RT-PCR screening of 116 samples targeting the L segment of the virus have revealed the positivity of two lung tissue homogenates originating from Murina aenea bat species. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M and L genome segments. The obtained results indicate the first molecular evidence for hantavirus in Murina aenae bat species and also the first discovery of a hantavirus in Murina bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests the identified virus may represent a novel species in Mobatvirus genus within Hantaviridae family. Furthermore, our results provide additional genomic data to help extend our knowledge about the evolution of these viruses.

Keywords

Mulu mobatvirus; MinION; Tb1-Lu; Mobatvirus; one health concept

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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