Preprint Article Version 1 This version is not peer-reviewed

Assessment of Probiotic Potential and Antagonistic Characteristics of Bifidobacterium bifidum Isolated from Infant Feces against Enteropathogenic Escherichia coli

Version 1 : Received: 8 July 2019 / Approved: 10 July 2019 / Online: 10 July 2019 (06:04:16 CEST)

How to cite: Ashraf, M.; Kanwal, R.; Khan, I.; Munir, S.; Aslam, R.; Ali, S.; Abbas, A.; Ali, M.; Abbas, G. Assessment of Probiotic Potential and Antagonistic Characteristics of Bifidobacterium bifidum Isolated from Infant Feces against Enteropathogenic Escherichia coli. Preprints 2019, 2019070137 (doi: 10.20944/preprints201907.0137.v1). Ashraf, M.; Kanwal, R.; Khan, I.; Munir, S.; Aslam, R.; Ali, S.; Abbas, A.; Ali, M.; Abbas, G. Assessment of Probiotic Potential and Antagonistic Characteristics of Bifidobacterium bifidum Isolated from Infant Feces against Enteropathogenic Escherichia coli. Preprints 2019, 2019070137 (doi: 10.20944/preprints201907.0137.v1).

Abstract

Enteropathogenic microorganisms like Escherichia coli cause severe intestinal problems by disrupting the gut homeostasis. The live microorganisms, when given in adequate quantities provide several beneficial effects to the host are known as probiotics. One of the pronounced benefits conferred by probiotic is to antagonize the growth of enteropathogens competing for adherence to the intestinal epithelium. Bifidobacterium is the major genera of human especially infant are intestinal microbiota. In current study, Bifidobacterium bifidum was isolated from the infant stools and probiotic potential was assessed using prescribed tolerance tests against low pH, gastric juices and bile salts. Anti-infectious activity of probiotic Bifidobacterium bifidum against enteropathogenic E. coli was checked both in vitro and in vivo using agar well diffusion assay and mice model respectively. Mice feces were evaluated for both Bifidobacterium bifidum and E. coli counts in all groups and analyzed statistically. In vitro results showed Bifidobacterium bifidum possess marked antibacterial activity against E. coli. There was significant decrease in enteropathogenic E. coli burden in the mice group fed with Bifidobacterium bifidum before and after challenge. In conclusion, the endogenous Bifidobacterium bifidum have excellent probiotic potential and can be used prophylactic and treatment option against enteropathogens.

Subject Areas

Escherichia coli; Bifidobacterium bifidum; probiotics; enteropathogens

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