Working Paper Article Version 1 This version is not peer-reviewed

FZD 10 Carried By Exosomes Sustains Cancer Cell Proliferation

Version 1 : Received: 8 July 2019 / Approved: 9 July 2019 / Online: 9 July 2019 (14:19:22 CEST)

A peer-reviewed article of this Preprint also exists.

Scavo, M.P.; Depalo, N.; Rizzi, F.; Ingrosso, C.; Fanizza, E.; Chieti, A.; Messa, C.; Denora, N.; Laquintana, V.; Striccoli, M.; Curri, M.L.; Giannelli, G. FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation. Cells 2019, 8, 777. Scavo, M.P.; Depalo, N.; Rizzi, F.; Ingrosso, C.; Fanizza, E.; Chieti, A.; Messa, C.; Denora, N.; Laquintana, V.; Striccoli, M.; Curri, M.L.; Giannelli, G. FZD10 Carried by Exosomes Sustains Cancer Cell Proliferation. Cells 2019, 8, 777.

Journal reference: Cells 2019, 8, 777
DOI: 10.3390/cells8080777

Abstract

Extracellular vesicles (EVs) are involved in intercellular communication during carcinogenesis and cancer cells are able to secrete EVs, in particular exosomes containing molecules, that can be transferred to recipient cells to induce pathological processes and significant modifications, as metastasis, increase of proliferation and carcinogenesis evolution. FZD proteins, a family of receptors comprised in the Wnt signaling pathway, play an important role in carcinogenesis of gastroenteric tract. Here, a still unrecognized role of Frizzled 10 (FZD10) protein was identified. In particular, the presence of FZD10 and FZD10-mRNA in exosomes extracted from culture medium of the untreated colorectal, gastric, hepatic and cholangio cancer cell lines, was detected. A substantial reduction in the FZD10 and FZD10-mRNA level was achieved in FZD10-mRNA silenced cells and in their corresponding exosomes and, concomitantly a significant decrease in viability of the silenced cells compared to their respective controls was observed. Interestingly, the incubation of silenced cells with exosomes extracted from culture medium of the same untreated cells promoted a remarkable restoration of the cell viability and, also, of the FZD10 and FZD10-mRNA level, thus indicating that the FZD10 and FZD10-mRNA delivering exosomes may be potential messengers of cancer reactivation and play an active role in long-distance metastatization

Subject Areas

Colorectal Cancer Cells; Gastric Cancer Cells; Cholangiocarcinoma Cells; Hepatocarcinoma Cells; Exosomes; FZD 10 protein; FZD10-mRNA; FZD10-mRNA Silenced Cells; Cell Proliferation

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