Preprint Review Version 1 This version is not peer-reviewed

MAPping Kinetochore MAP Function Required for Stabilizing Microtubule Attachments to Chromosomes during Metaphase

Version 1 : Received: 5 June 2019 / Approved: 10 June 2019 / Online: 10 June 2019 (11:00:18 CEST)

How to cite: Varma, D.; Amin, M.; Agarwal, S. MAPping Kinetochore MAP Function Required for Stabilizing Microtubule Attachments to Chromosomes during Metaphase. Preprints 2019, 2019060076 (doi: 10.20944/preprints201906.0076.v1). Varma, D.; Amin, M.; Agarwal, S. MAPping Kinetochore MAP Function Required for Stabilizing Microtubule Attachments to Chromosomes during Metaphase. Preprints 2019, 2019060076 (doi: 10.20944/preprints201906.0076.v1).

Abstract

In mitosis, faithful chromosome segregation is orchestrated by the dynamic interactions between the spindle microtubules (MTs) emanating from the opposite poles and the kinetochores of chromosomes. However, the precise mechanism that coordinates the coupling of kinetochore components to dynamic MTs has been a long-standing question. Microtubule (MT) associated proteins (MAPs) regulate MT nucleation, dynamics, MT-mediated transport and MT cross-linking in cells. Especially during mitosis, MAPs play an essential role not only in determining the spindle length, position and orientation but also in facilitating robust kinetochore-microtubule (kMT) attachments by linking the kinetochores to spindle MTs efficiently. MT-stability imparted by the MAPs is critical to ensure accurate chromosome segregation. This review primarily focuses on the specific function of non-motor kinetochore MAPs, their recruitment to kinetochores and their MT-binding properties. We also attempt to synthesize and strengthen our understanding of how these MAPs work in coordination with the kinetochore-bound Ndc80 complex (the key component of the MT-binding interface in metaphase and anaphase) to establish stable kMT attachments and control accurate chromosome segregation during mitosis.

Subject Areas

microtubules; kinetochores; mitosis; chromosomes; MAPs; ndc80

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