Version 1
: Received: 26 May 2019 / Approved: 27 May 2019 / Online: 27 May 2019 (12:56:49 CEST)
How to cite:
Hasan, M.M.; Hasan, M.K.; Rashid, M.H.; Khayer, A. Isolation of Protease Inhibitors from Cyanobacteriaum Microcystis sp. Strain BM 25 Which Inhibits Major Digestive Enzyme in Daphnia magna. Preprints2019, 2019050319. https://doi.org/10.20944/preprints201905.0319.v1
Hasan, M.M.; Hasan, M.K.; Rashid, M.H.; Khayer, A. Isolation of Protease Inhibitors from Cyanobacteriaum Microcystis sp. Strain BM 25 Which Inhibits Major Digestive Enzyme in Daphnia magna. Preprints 2019, 2019050319. https://doi.org/10.20944/preprints201905.0319.v1
Hasan, M.M.; Hasan, M.K.; Rashid, M.H.; Khayer, A. Isolation of Protease Inhibitors from Cyanobacteriaum Microcystis sp. Strain BM 25 Which Inhibits Major Digestive Enzyme in Daphnia magna. Preprints2019, 2019050319. https://doi.org/10.20944/preprints201905.0319.v1
APA Style
Hasan, M.M., Hasan, M.K., Rashid, M.H., & Khayer, A. (2019). Isolation of Protease Inhibitors from Cyanobacteriaum <em>Microcystis </em>sp. Strain BM 25 Which Inhibits Major Digestive Enzyme in <em>Daphnia magna</em>. Preprints. https://doi.org/10.20944/preprints201905.0319.v1
Chicago/Turabian Style
Hasan, M.M., M. Harun-or Rashid and Abul Khayer. 2019 "Isolation of Protease Inhibitors from Cyanobacteriaum <em>Microcystis </em>sp. Strain BM 25 Which Inhibits Major Digestive Enzyme in <em>Daphnia magna</em>" Preprints. https://doi.org/10.20944/preprints201905.0319.v1
Abstract
Cyanobacterial mass developments in eutrophic ponds and lakes are a major concern for lake management, as many cyanobacteria produce a huge variety of toxic secondary metabolites, e.g. microcystins. The aim of this research was to culture a strain of the cyanobacterium Microcystis sp strain BM25, to observe its biomass production and to isolate and purify protease inhibitors from this cyanobacterial biomass. Different secondary metabolites were isolated following a standard bioassay-guideline. Isolation was performed, with an enzymatic protease assay as bioassay. High performance liquid chromatography was used to identify different fractions of secondary metabolite from the strain BM25. Moreover, protease homogenates were isolated from Daphnia magna in order to test the inhibitors against naturally occurring major digestive proteases trypsin and chymotrypsin. It was measured that 60% MeOH and the 80% MeOH C18-SPE fraction inhibits chymotrypsin activity 98% (6 nmol pNA min-1 mg-1) and 99 % (4 nmol pNA min-1 mg-1), respectively. In contrast, trypsin activity was not inhibited by methanolic extracts of this cyanobacterium strain.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
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The commenter has declared there is no conflict of interests.