Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Antagonistic Interactions between Benzo[a]pyrene and C60 in Toxicological Response of Marine Mussels

Audrey Barranger
ORCID logo ,
Laura M. Langan
,
Vikram Sharma
,
Graham A. Rance
ORCID logo ,
Yann Aminot
,
Nicola J. Weston
,
Farida Akcha
,
Michael N. Moore
,
Volker M. Arlt
ORCID logo ,
Andrei N. Khlobystov
,
James W. Readman
,
Awadhesh N. Jha
* ORCID logo
Version 1 : Received: 10 May 2019 / Approved: 15 May 2019 / Online: 15 May 2019 (09:57:16 CEST)

A peer-reviewed article of this Preprint also exists.

Barranger, A.; Langan, L.M.; Sharma, V.; Rance, G.A.; Aminot, Y.; Weston, N.J.; Akcha, F.; Moore, M.N.; Arlt, V.M.; Khlobystov, A.N.; Readman, J.W.; Jha, A.N. Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels. Nanomaterials 2019, 9, 987. Barranger, A.; Langan, L.M.; Sharma, V.; Rance, G.A.; Aminot, Y.; Weston, N.J.; Akcha, F.; Moore, M.N.; Arlt, V.M.; Khlobystov, A.N.; Readman, J.W.; Jha, A.N. Antagonistic Interactions between Benzo[a]pyrene and Fullerene (C60) in Toxicological Response of Marine Mussels. Nanomaterials 2019, 9, 987.

Abstract

This study aimed to assess the ecotoxicological effects of the interaction of fullerene (C60) and benzo[a]pyrene (B[a]P) on the marine mussel, Mytilus galloprovincialis. The uptake of nC60, B[a]P and mixtures of nC60 and B[a]P into tissues was confirmed by GC-MS, LC-HRMS and ICP-MS. Biomarkers of DNA damage as well as proteomics analysis were applied to unravel the toxic effect of B[a]P and C60. Antagonistic responses were observed at the genotoxic and proteomic level. Differentially expressed proteins (DEPs) were only identified in the B[a]P single exposure and the B[a]P mixture exposure groups containing 1 mg/L of C60, the majority of which were down-regulated (~52%). No DEPs were identified at any of the concentrations of nC60 (p < 0.05, 1% FDR). Using DEPs identified at a threshold of (p < 0.05; B[a]P and B[a]P mixture with nC60), gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis indicated that these proteins were enriched with a broad spectrum of biological processes and pathways, including those broadly associated with protein processing, cellular processes and environmental information processing. Among those significantly enriched pathways, the ribosome was consistently the top enriched term irrespective of treatment or concentration and plays an important role as the site of biological protein synthesis and translation. Our results demonstrate the complex multi-modal response to environmental stressors in M. galloprovincialis.

Keywords

Trojan Horse effect; B[a]P; nC60; co-exposure; Mussels; DNA damage; proteomics

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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