Preprint Review Version 1 This version is not peer-reviewed

Methods to Grow better Diffractive Protein Crystals Acquired through Space Experiments

Version 1 : Received: 10 May 2019 / Approved: 13 May 2019 / Online: 13 May 2019 (01:47:52 CEST)

How to cite: Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods to Grow better Diffractive Protein Crystals Acquired through Space Experiments. Preprints 2019, 2019050140 (doi: 10.20944/preprints201905.0140.v1). Hashizume, Y.; Inaka, K.; Furubayashi, N.; Kamo, M.; Takahashi, S.; Tanaka, H. Methods to Grow better Diffractive Protein Crystals Acquired through Space Experiments. Preprints 2019, 2019050140 (doi: 10.20944/preprints201905.0140.v1).

Abstract

We summarize how to obtain protein crystals from which better diffraction images can be obtained. In particular, the quality evaluation of the protein sample, the crystallization method and crystallization conditions, the freezing protection of the crystal, and the crystallization in the microgravity environment are described in detail.

Subject Areas

protein crystallization; protein sample qualification; JAXA PCG; microgravity

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