Working Paper Article Version 1 This version is not peer-reviewed

Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas

Version 1 : Received: 29 April 2019 / Approved: 3 May 2019 / Online: 3 May 2019 (13:59:22 CEST)

A peer-reviewed article of this Preprint also exists.

Hitte, C.; Le Béguec, C.; Cadieu, E.; Wucher, V.; Primot, A.; Prouteau, A.; Botherel, N.; Hédan, B.; Lindblad-Toh, K.; André, C.; Derrien, T. Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas. Genes 2019, 10, 477. Hitte, C.; Le Béguec, C.; Cadieu, E.; Wucher, V.; Primot, A.; Prouteau, A.; Botherel, N.; Hédan, B.; Lindblad-Toh, K.; André, C.; Derrien, T. Genome-Wide Analysis of Long Non-Coding RNA Profiles in Canine Oral Melanomas. Genes 2019, 10, 477.

Journal reference: Genes 2019, 10, 477
DOI: 10.3390/genes10060477

Abstract

Mucosal melanomas (MM) are rare aggressive cancers in humans and one of the most common forms of oral cancers in dogs. Similar biological and histological features are shared between MM in both species making dogs a powerful model for comparative oncology studies of melanomas. Although exome sequencing recently identified recurrent coding mutations in canine MM, little is known about changes in non-coding gene expression and more particularly in canine long non-coding RNAs (lncRNAs), which are commonly dysregulated in human cancers. Here, we sampled a large cohort (n= 52) of canine normal/tumor oral MM from three predisposed breeds (poodles, Labrador retrievers and golden retrievers) and used deep transcriptome sequencing to identify more than 400 differentially expressed (DE) lncRNAs. We further prioritized candidate lncRNAs by comparative genomic analysis to pinpoint 26 dog-human conserved DE lncRNAs, including SOX21-AS, ZEB2-AS and CASC15 lncRNAs. Using unsupervised co-expression networks analysis with coding genes, we inferred potential functions of DE lncRNAs suggesting associations with cancer-related genes, cell cycle and carbohydrate metabolism GO terms. Finally, we exploited our multi-breed design to identify DE lncRNAs per breed. This study provides a unique transcriptomic resource for studying oral melanoma in dogs and highlights lncRNAs that may potentially be diagnostic or therapeutic targets for human and veterinary medicine.

Subject Areas

mucosal melanoma; dogs; transcriptome sequencing; long non-coding RNAs (lncRNAs)

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