Communication
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Extracellular Vesicle Integrins Distinguish Unique Cancers
Version 1
: Received: 3 April 2019 / Approved: 4 April 2019 / Online: 4 April 2019 (12:29:44 CEST)
A peer-reviewed article of this Preprint also exists.
Hurwitz, S.N.; Meckes, D.G., Jr. Extracellular Vesicle Integrins Distinguish Unique Cancers. Proteomes 2019, 7, 14. Hurwitz, S.N.; Meckes, D.G., Jr. Extracellular Vesicle Integrins Distinguish Unique Cancers. Proteomes 2019, 7, 14.
Abstract
The proteomic profile of extracellular vesicles (EVs) has been of increasing interest, particularly in understanding cancer growth, drug resistance, and metastatic behavior. Emerging data suggests that cancer-derived EVs may carry an array of oncogenic cargo, including certain integrin proteins that may, in turn, promote cell detachment, migration, and selection of future metastatic sites. We previously reported a large comparison of secreted vesicle protein cargo across sixty diverse human cancer cell lines. Here, we analyze the distinct integrin profiles of these cancer EVs. We further demonstrate the enrichment of integrin receptors in breast cancer EVs compared to vesicles secreted from benign breast epithelial cells. Total EV integrin levels, including the quantity of integrins α2, αv, β4, and β5 correlate with breast tumor stage. In particular, integrin α2 also largely reflects progenitor cell expression, highlighting the utility of this integrin protein as a potential circulating biomarker of primary tumors. This study provides preliminary evidence of the value of vesicle-associated integrin proteins in cancer diagnosis and prediction of tumor stage. Differential expression of integrins across cancer cells, and selective packaging of integrins into EVs may contribute to further understanding the development and progression of tumor growth and metastasis across a variety of cancer types.
Keywords
Exosomes, mass spectrometry, proteomics, biomarkers, cancer, extracellular vesicles, microvesicles, oncosomes
Subject
Medicine and Pharmacology, Oncology and Oncogenics
Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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