Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Interscapular and Perivascular Brown Adipose Tissue Respond Differently to a Short-Term High-Fat Diet

Peter Aldiss
,
Jo Lewis
ORCID logo ,
David Boocock
ORCID logo ,
Amanda Miles
ORCID logo ,
Ian Bloor
,
Francis Ebling
,
Helen Budge
,
Michael Symonds
* ORCID logo
Version 1 : Received: 20 February 2019 / Approved: 21 February 2019 / Online: 21 February 2019 (10:01:04 CET)

A peer-reviewed article of this Preprint also exists.

Aldiss, P.; Symonds, M.E.; Lewis, J.E.; Boocock, D.J.; Miles, A.K.; Bloor, I.; Ebling, F.J.P.; Budge, H. Interscapular and Perivascular Brown Adipose Tissue Respond Differently to a Short-Term High-Fat Diet. Nutrients 2019, 11, 1065. Aldiss, P.; Symonds, M.E.; Lewis, J.E.; Boocock, D.J.; Miles, A.K.; Bloor, I.; Ebling, F.J.P.; Budge, H. Interscapular and Perivascular Brown Adipose Tissue Respond Differently to a Short-Term High-Fat Diet. Nutrients 2019, 11, 1065.

Abstract

Brown adipose tissue (BAT) function may depend on its anatomical location and developmental origin. Interscapular BAT (iBAT) regulates acute macronutrient metabolism, whilst perivascular BAT (PVAT) regulates vascular function. Although phenotypically similar, whether these depots respond differently to acute nutrient excess is unclear. Given their distinct anatomical locations and developmental origins and we hypothesised that iBAT and PVAT would respond differently to brief period of nutrient excess. Sprague-Dawley rats aged 12 weeks (n = 12) were fed either a standard (10% fat, n = 6) or high fat diet (HFD: 45% fat, n = 6) for 72 h and housed at thermoneutrality. Following an assessment of whole body physiology, fat was collected from both depots for analysis of gene expression and the proteome. HFD consumption for 72 h induced rapid weight gain (c. 2.6%) and reduced serum NEFA with no change in either total adipose or depot mass. In iBAT, an upregulation of genes involved in insulin signalling and lipid metabolism was accompanied by enrichment of lipid-related processes and functions, plus glucagon and PPAR signalling pathways. In PVAT, HFD induced a pronounced down-regulation of multiple metabolic pathways which was accompanied with increased abundance of proteins involved in apoptosis (e.g. Hdgf and Ywaq) and toll-like receptor signalling (Ube2n). There was also an enrichment of DNA-related processes and functions (e.g., nucleosome assembly and histone exchange) and RNA degradation and cell adhesion pathways. In conclusion, we show that iBAT and PVAT elicit divergent responses to short-term nutrient excess highlighting early adaptations in these depots before changes in fat mass.

Keywords

brown fat; white fat; proteome; nutrient excess

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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