Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Standardized in vivo Evaluation of Biocompatibility and Bioresorption of a New Synthetic Membrane for Guided Bone Regeneration

Lívia da Costa Pereira
,
Carlos Fernando de Almeida Barros Mourão
ORCID logo ,
Adriana Terezinha Neves Novellino Alves
,
Rodrigo Figueiredo de Brito Resende
,
Marcelo José Pinheiro Guedes de Uzeda
,
Rafael Seabra Louro
,
Mônica Diuana Calasans-Maia
*
Version 1 : Received: 25 January 2019 / Approved: 29 January 2019 / Online: 29 January 2019 (09:38:04 CET)

A peer-reviewed article of this Preprint also exists.

da Costa Pereira, L.; de Almeida Barros Mourão, C.F.; Neves Novellino Alves, A.T.; Figueiredo de Brito Resende, R.; Pinheiro Guedes de Uzeda, M.J.; Granjeiro, J.M.; Seabra Louro, R.; Diuana Calasans-Maia, M. In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration. Materials 2019, 12, 1186. da Costa Pereira, L.; de Almeida Barros Mourão, C.F.; Neves Novellino Alves, A.T.; Figueiredo de Brito Resende, R.; Pinheiro Guedes de Uzeda, M.J.; Granjeiro, J.M.; Seabra Louro, R.; Diuana Calasans-Maia, M. In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration. Materials 2019, 12, 1186.

Abstract

This study aimed to evaluate the physico-chemical properties, biocompatibility and bioabsorption of 3 different new membranes for bone guided regeneration (PLGA associated with hydroxyapatite and beta-TCP) with three thicknesses (200, 500 and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and the quantification of Carbon, Hidrogen and Nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1 - Sham (without implantation); Group 2 - 200 μm; Group 3 - 500 μm; Group 4 - 700 μm; and Group 5 - Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions in the experimental periods studied. The 500 μm membrane did not show tissue reaction for any experimental periods studied. The 200 μm membrane membranes began to exhibit fragmentation after 30- day, while the 500 and 700 µm membranes started the fragmentation at 90-day. All membranes studied were biocompatible, and the 500µm membrane showed the best results being a promissory membrane for bone guided regeneration.

Keywords

Membranes; Polymer; Biocompatibility; Inflammatory cells; Calcium Phosphate

Subject

Medicine and Pharmacology, Dentistry and Oral Surgery

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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