Preprint Article Version 1 This version is not peer-reviewed

Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents

Version 1 : Received: 27 December 2018 / Approved: 28 December 2018 / Online: 28 December 2018 (12:34:36 CET)
Version 2 : Received: 11 January 2019 / Approved: 14 January 2019 / Online: 14 January 2019 (12:21:03 CET)

How to cite: Kovel, E.; Sachkova, A.; Vnukova, N.; Churilov, G.; Knyazeva, E.; Kudryasheva, N. Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents. Preprints 2018, 2018120348 (doi: 10.20944/preprints201812.0348.v1). Kovel, E.; Sachkova, A.; Vnukova, N.; Churilov, G.; Knyazeva, E.; Kudryasheva, N. Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents. Preprints 2018, 2018120348 (doi: 10.20944/preprints201812.0348.v1).

Abstract

Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. We studied biological effects of a series of fullerenols. Antioxidant activity and toxicity of the fullerenols were compared using bioluminescence assays (cellular and enzymatic); a content of Reactive Oxygen Species in fullerenol solutions was determined using chemiluminescence luminol method. Two groups of fullerenols with different number of hydroxyl substituents were under investigation: (I) С60Оy(OH)x, С60,70Оy(OH)x, where х+у=24–28 and (II) С60,70Оy(OH)x, Fe0,5С60Оy(OH)x, where х+у=40–42. Toxicity of the fullerenols was evaluated using effective concentrations ЕС50. Fullerenol’ antioxidant activity was investigated in model solutions of organic toxicant of oxidative type, 1,4-benzoquinone. Detoxification coefficients were calculated to analyze and compare the antioxidant activity. Higher toxicity and lower antioxidant activity were demonstrated in the solutions of fullerenols with higher number of the oxygen substituents (х+у=40–42). The differences were concerned with fullerenol’ ability to disturb Reactive Oxygen Species balance in aqueous solutions. Toxic effect of the prospective endohedral metal-fullerenol with gadolinium atom involved, [email protected](OH)x, where х+у=40–42, was evaluated and explained by a high number of oxygen groups

Subject Areas

bioactive compound; fullerenol; antioxidant activity; toxicity; reactive oxygen species; bioluminescence bioassay

Readers' Comments and Ratings (0)

Leave a public comment
Send a private comment to the author(s)
Rate this article
Views 0
Downloads 0
Comments 0
Metrics 0
Leave a public comment

×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.