Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

Discovering Seed Proteome: Challenges and Perspectives

Galina Smolikova
,
Elena Lukasheva
ORCID logo ,
Gregory Mavropolo-Stolyarenko
,
Tatiana Bilova
ORCID logo ,
Alena Soboleva
,
Alexander Tsarev
,
Ekaterina Podolskaya
,
Vladimir Zhukov
,
Igor Tikhonovich
,
Sergei Medvedev
,
Wolfgang Hoehenwarter
ORCID logo ,
Andrej Frolov
*
Version 1 : Received: 7 December 2018 / Approved: 11 December 2018 / Online: 11 December 2018 (11:00:26 CET)

How to cite: Smolikova, G.; Lukasheva, E.; Mavropolo-Stolyarenko, G.; Bilova, T.; Soboleva, A.; Tsarev, A.; Podolskaya, E.; Zhukov, V.; Tikhonovich, I.; Medvedev, S.; Hoehenwarter, W.; Frolov, A. Discovering Seed Proteome: Challenges and Perspectives. Preprints 2018, 2018120129. https://doi.org/10.20944/preprints201812.0129.v1 Smolikova, G.; Lukasheva, E.; Mavropolo-Stolyarenko, G.; Bilova, T.; Soboleva, A.; Tsarev, A.; Podolskaya, E.; Zhukov, V.; Tikhonovich, I.; Medvedev, S.; Hoehenwarter, W.; Frolov, A. Discovering Seed Proteome: Challenges and Perspectives. Preprints 2018, 2018120129. https://doi.org/10.20944/preprints201812.0129.v1

Abstract

For centuries, crop plants have represented the basis of the daily human diet. Among them, cereals and legumes, accumulating oils, proteins and carbohydrates in their seeds, distinctly dominate modern agronomic practice. Indeed, these plants play an essential role in the food industry and fuel production. Therefore, the seeds of crop plants are intensively studied by food chemists, biologists, biochemists, and nutritional physiologists. Accordingly, not only seed development and germination, but also age- and stress-related alterations in seed vigor, longevity, nutritional value and safety can be addressed by a broad panel of analytical, biochemical and physiological methods. Currently, functional genomics is one of the most powerful tools, giving direct access to characteristic metabolic changes, accompanying plant development, senescence and response to biotic or environmental stress. Among individual methodological platforms, proteomics represents one of the most effective ones, giving access to cellular metabolism at the level of proteins. Here we discuss the main methodological approaches employed by seed proteomics in the context of physiological changes related to seed development, ageing and response to environmental stress.

Keywords

food safety; gel-based proteomics; LC-based proteomics; post-translational modifications; proteomics; seed ageing; seed quality

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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