Preprint Article Version 1 This version is not peer-reviewed

Anti-Inflammatory Activity of Quercetogetin Isolated from Citrus Unshiu Peel Involves Suppression of NF-κB and MAPK Signaling Pathways in LPS-induced RAW264.7 Macrophages

Version 1 : Received: 7 November 2018 / Approved: 9 November 2018 / Online: 9 November 2018 (03:31:28 UTC)

How to cite: Son, E.S.; Park, J.; Park, H.R.; Han, W.; Yun, D.E.; Kwon, O.C.; Nam, J.Y.; Park, Y.J.; Lee, C.S. Anti-Inflammatory Activity of Quercetogetin Isolated from Citrus Unshiu Peel Involves Suppression of NF-κB and MAPK Signaling Pathways in LPS-induced RAW264.7 Macrophages. Preprints 2018, 2018110229 (doi: 10.20944/preprints201811.0229.v1). Son, E.S.; Park, J.; Park, H.R.; Han, W.; Yun, D.E.; Kwon, O.C.; Nam, J.Y.; Park, Y.J.; Lee, C.S. Anti-Inflammatory Activity of Quercetogetin Isolated from Citrus Unshiu Peel Involves Suppression of NF-κB and MAPK Signaling Pathways in LPS-induced RAW264.7 Macrophages. Preprints 2018, 2018110229 (doi: 10.20944/preprints201811.0229.v1).

Abstract

Citrus peel has been used in Asian traditional medicine for the treatment of cough, asthma, and bronchial disorders. However, the anti-inflammatory effect of quercetogetin (QUE), a polymethoxylated flavone isolated from the peel of citrus unshui is poorly understood. We investigated the anti-inflammatory effect and the molecular mechanisms of QUE in lipopolysaccharide (LPS)-induced RAW264.7 cells. QUE inhibited the production of NO and prostaglandin E2 by suppressing the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at both the mRNA and protein levels. QUE suppressed the production of proinflammatory cytokines, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. QUE also inhibited the translocation of the nuclear factor kappa B subunit, p65, into the nucleus by interrupting the phosphorylation of IκB-α in LPS-induced RAW 264.7 cells. Based on the finding that QUE significantly decreased p-ERK protein expression in LPS-induced RAW264.7 cells, we confirmed that suppression of the inflammatory process by QUE was mediated through the MAPK pathway. This is the first report on the strong anti-inflammatory effects of QUE, which is a compound that can potentially be used as a therapeutic agent for inflammatory diseases.

Subject Areas

quercetogetin (QUE); anti-inflammatory; inducible nitric oxide synthase (iNOS); cyclooxygenase-2 (COX-2); MAPK; NF-κB

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