Preprint Article Version 1 This version is not peer-reviewed

Characterization of Microbiota in Bronchiectasis Patients with Different Disease Severities

Version 1 : Received: 10 October 2018 / Approved: 10 October 2018 / Online: 10 October 2018 (08:14:24 CEST)

A peer-reviewed article of this Preprint also exists.

Lee, S.H.; Lee, Y.; Park, J.S.; Cho, Y.-J.; Yoon, H.I.; Lee, C.-T.; Lee, J.H. Characterization of Microbiota in Bronchiectasis Patients with Different Disease Severities. J. Clin. Med. 2018, 7, 429. Lee, S.H.; Lee, Y.; Park, J.S.; Cho, Y.-J.; Yoon, H.I.; Lee, C.-T.; Lee, J.H. Characterization of Microbiota in Bronchiectasis Patients with Different Disease Severities. J. Clin. Med. 2018, 7, 429.

Journal reference: J. Clin. Med. 2018, 7, 429
DOI: 10.3390/jcm7110429

Abstract

The application of 16S rRNA gene pyrosequencing has expanded our knowledge about the respiratory tract microbiome originally obtained using conventional, culture-based methods. In this study, we employed DNA-based molecular techniques for examining the sputum microbiome in bronchiectasis patients in relation to disease severity. Of 63 study subjects, 42 had mild and 21 had moderate or severe bronchiectasis, which was classified by calculating the FACED score based on FEV1 (forced expiratory volume in 1 s, %) (F, 0–2 points), age (A, 0–2 points), chronic colonization by Pseudomonas aeruginosa (C, 0-1 point), radiographic extension (E, 0–1 point), and dyspnoea (D, 0–1 point). Bronchiectasis was defined as mild at 0–2 points, moderate at 3–4 points, and severe at 5–7 points. The mean age was 68.0 ± 9.3 years; 33 patients were women. Haemophilus (p=0.005) and Rothia (p=0.043) were significantly more abundant in the mild bronchiectasis group, whereas Pseudomonas (p=0.031) was significantly more abundant in the moderate or severe group. However, the alpha or beta diversity did not significantly differ among sputum microbiota, i.e. the same dominating genera were found in all samples. Further large-scale studies are needed to investigate the sputum microbiome in bronchiectasis.

Subject Areas

bronchiectasis; FACED score; microbiome

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.