Preprint Article Version 2 This version is not peer-reviewed

Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models

Version 1 : Received: 4 October 2018 / Approved: 4 October 2018 / Online: 4 October 2018 (16:23:44 CEST)
Version 2 : Received: 5 December 2018 / Approved: 5 December 2018 / Online: 5 December 2018 (16:14:52 CET)

How to cite: Percival, B.; Grootveld, M.; Gibson, M.; Osman, Y.; Molinari, M.; Jafari, F.; Sahota, T..; Martin, M.; Casanova, F.; Mather, M.; Edgar, M.; Masania, J..; Wilson, P. Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models. Preprints 2018, 2018100089 Percival, B.; Grootveld, M.; Gibson, M.; Osman, Y.; Molinari, M.; Jafari, F.; Sahota, T..; Martin, M.; Casanova, F.; Mather, M.; Edgar, M.; Masania, J..; Wilson, P. Low-Field, Benchtop NMR Spectroscopy as a Potential Tool for Point-of-Care Diagnostics of Metabolic Conditions: Validation, Protocols and Computational Models. Preprints 2018, 2018100089

Abstract

Novel sensing technologies for liquid biopsies offer a promising prospect for the early detection of metabolic conditions through -omics techniques. Indeed, high-field NMR facilities are routinely used for metabolomics investigations on a range of biofluids in order to rapidly recognize unusual metabolic patterns in patients suffering from a range of diseases. However, these techniques are restricted by the prohibitively large size and cost of such facilities, suggesting a possible role for smaller, low-field NMR instruments in biofluid analysis. Herein we describe selected biomolecule validation on a low-field benchtop NMR spectrometer (60 MHz), and present an associated protocol for the analysis of biofluids on compact NMR instruments. We successfully detect common markers of diabetic control at low-to-medium concentrations through optimized experiments, including glucose (≤ 2.6 mmol./L) and acetone (25 μmol./L), and additionally in readily-accessible biofluids. We present a combined protocol for the analysis of these biofluids with low-field NMR spectrometers for metabolomics, and offer a perspective on the future of this technique appealing to point-of-care applications.

Subject Areas

Metabolomics; Benchtop NMR; Biomarkers; Biomolecules; Validation; Protocol

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