Preprint Article Version 1 This version is not peer-reviewed

A Distinct Pattern of Circulating Amino Acids Characterizes Older Persons with Physical Frailty and Sarcopenia: Preliminary Results from the BIOSPHERE Study

Version 1 : Received: 12 September 2018 / Approved: 12 September 2018 / Online: 12 September 2018 (17:11:33 CEST)

How to cite: Calvani, R.; Picca, A.; Marini, F.; Biancolillo, A.; Gervasoni, J.; Persichilli, S.; Primiano, A.; Coelho-Junior, H.; Bossola, M.; Urbani, A.; Landi, F.; Bernabei, R.; Marzetti, E. A Distinct Pattern of Circulating Amino Acids Characterizes Older Persons with Physical Frailty and Sarcopenia: Preliminary Results from the BIOSPHERE Study. Preprints 2018, 2018090224 (doi: 10.20944/preprints201809.0224.v1). Calvani, R.; Picca, A.; Marini, F.; Biancolillo, A.; Gervasoni, J.; Persichilli, S.; Primiano, A.; Coelho-Junior, H.; Bossola, M.; Urbani, A.; Landi, F.; Bernabei, R.; Marzetti, E. A Distinct Pattern of Circulating Amino Acids Characterizes Older Persons with Physical Frailty and Sarcopenia: Preliminary Results from the BIOSPHERE Study. Preprints 2018, 2018090224 (doi: 10.20944/preprints201809.0224.v1).

Abstract

Physical frailty and sarcopenia (PF&S) are hallmarks of aging that share a common pathogenic background. Perturbations in protein/amino acid metabolism may play a role in the development of PF&S. In this preliminary study, 68 community-dwellers aged 70 years and older, 38 with PF&S and 30 non-sarcopenic, non-frail controls (nonPF&S), were enrolled. A panel of 37 serum amino acids and derivatives was assayed by UPLC-MS. Partial Least Squares Discriminant Analysis (PLS-DA) was used to characterize the amino acid profile of PF&S. The optimal complexity of the PLS-DA model was found to be three latent variables. The proportion of correct classification was 76.6 ± 3.9% (75.1 ± 4.6% for enrollees with PF&S; 78.5 ± 6.0% for controls). Older adults with PF&S were characterized by higher levels of asparagine, aspartic acid, citrulline, ethanolamine, glutamic acid, sarcosine, and taurine. The profile of nonPF&S individuals was defined by higher levels of α-aminobutyric acid and methionine. Distinct profiles of circulating amino acids and derivatives characterize older individuals with PF&S. The dissection of these patterns may provide novel insights into the role played by protein/amino acid perturbations in the disabling cascade and possible new targets for interventions.

Subject Areas

aging; muscle; protein; metabolism; metabolomics; profiling; biomarkers; multi-marker; physical performance; multivariate

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