Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Developing Sensitive Golden Plate Biosensor for Early Detection of Serum Cardiac Troponin I as Diagnostic Biomarkers

Said Moselhy
* ,
Ehab Mostafa
,
Abdulrahman Almalki
,
Taha Kumosani
,
Youssri Soliman
,
Ibrahim Aboseda
,
Wesam Abduaal
,
Rwaa Abdulaal
,
Numan Salah
,
Walid Alshawafi
,
Afnan Kunosani
Version 1 : Received: 5 September 2018 / Approved: 5 September 2018 / Online: 5 September 2018 (10:09:33 CEST)

How to cite: Moselhy, S.; Mostafa, E.; Almalki, A.; Kumosani, T.; Soliman, Y.; Aboseda, I.; Abduaal, W.; Abdulaal, R.; Salah, N.; Alshawafi, W.; Kunosani, A. Developing Sensitive Golden Plate Biosensor for Early Detection of Serum Cardiac Troponin I as Diagnostic Biomarkers. Preprints 2018, 2018090093. https://doi.org/10.20944/preprints201809.0093.v1 Moselhy, S.; Mostafa, E.; Almalki, A.; Kumosani, T.; Soliman, Y.; Aboseda, I.; Abduaal, W.; Abdulaal, R.; Salah, N.; Alshawafi, W.; Kunosani, A. Developing Sensitive Golden Plate Biosensor for Early Detection of Serum Cardiac Troponin I as Diagnostic Biomarkers. Preprints 2018, 2018090093. https://doi.org/10.20944/preprints201809.0093.v1

Abstract

Rapid diagnosis of acute myocardial infarction is critical for intervention and treatment to decrease morbidity and mortality. For this reasons, this study aimed to design a sensitive biosensor for cardiac troponin I (cTnI) for early prediction and intervention. The sensor was designed from golden plate immobilized with anti- cTnI antibody conjugated with horseradish peroxidase (HRP) enzyme. The rate of degradation of H2O2 is directly related to serum cTnI level. Serum cTnI level was quantified from 3 studied groups including; healthy subjects and patients with ischemic heart (IHD) diseases and myocardial infarction (MI) by ELISA and biosensor techniques to achieve the improved sensitivity of the biosensor. It was found that cTnI levels in healthy subjects ranged from 8-13 ng/dl and in patients with IHD ranged from 20 - 41 ng/dl and MI range from 57- 180 ng/dl. The sensitivity of biosensor was ranged from 85-95% compared with ELISA technique. The developed biosensor is promising in ICU hospitals for early diagnosis and prevent complications of the stroke. It is recommended to apply this biosensor in the large scale of patients with different stages of the cardiac disease.

Keywords

Troponin I- Myocardial infarction- Ischemic heart diseases- Biosensors- golden plate.

Subject

Biology and Life Sciences, Anatomy and Physiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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