Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon

Sonal Gupta
ORCID logo ,
Nidhi Gupta
,
Pradeep Tiwari
,
Saji Menon
,
Praveen Mathur
,
Shanker Lal Kothari
,
Sivaramaiah Nallapeta
,
Krishna Mohan Medicherla
* ORCID logo ,
Prashanth Suravajhala
* ORCID logo
Version 1 : Received: 30 August 2018 / Approved: 30 August 2018 / Online: 30 August 2018 (15:30:35 CEST)

A peer-reviewed article of this Preprint also exists.

Gupta, S.; Gupta, N.; Tiwari, P.; Menon, S.; Mathur, P.; Kothari, S.L.; Nallapeta, S.; Medicherla, K.M.; Suravajhala, P. Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon. Biomolecules 2018, 8, 95. Gupta, S.; Gupta, N.; Tiwari, P.; Menon, S.; Mathur, P.; Kothari, S.L.; Nallapeta, S.; Medicherla, K.M.; Suravajhala, P. Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon. Biomolecules 2018, 8, 95.

Abstract

Congenital Pouch Colon (CPC) is a rare anorectal anomaly common to North Western India specifically Rajasthan. Despite efforts to understand the clinical genetic makeup of CPC, no attempt on identifying non-coding RNAs was done. We have earlier reported CPC's rare variants from whole exome sequencing across 18 affected samples in a total of 64 subjects. A Smith-Waterman algorithm was used to infer a couple of lncRNAs from WES samples of CPC with predictions from the Noncode database. Further screening and quantification using PCR, we ascertained interactions using Micro Scale Thermophoresis (MST). We report the role of lnc-EPB41-1-1 shown to be promiscuously interacting with KIF13A substantiating their role in regulation.

Keywords

long non coding RNA, whole exome sequencing, protein interaction, congenital pouch colon, microscale thermophoresis

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.