Preprint Communication Version 1 This version is not peer-reviewed

lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon

Version 1 : Received: 30 August 2018 / Approved: 30 August 2018 / Online: 30 August 2018 (15:30:35 CEST)

A peer-reviewed article of this Preprint also exists.

Gupta, S.; Gupta, N.; Tiwari, P.; Menon, S.; Mathur, P.; Kothari, S.L.; Nallapeta, S.; Medicherla, K.M.; Suravajhala, P. Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon. Biomolecules 2018, 8, 95. Gupta, S.; Gupta, N.; Tiwari, P.; Menon, S.; Mathur, P.; Kothari, S.L.; Nallapeta, S.; Medicherla, K.M.; Suravajhala, P. Lnc-EPB41-Protein Interactions Associated with Congenital Pouch Colon. Biomolecules 2018, 8, 95.

Journal reference: Biomolecules 2018, 8, 95
DOI: 10.3390/biom8030095

Abstract

Congenital Pouch Colon (CPC) is a rare anorectal anomaly common to North Western India specifically Rajasthan. Despite efforts to understand the clinical genetic makeup of CPC, no attempt on identifying non-coding RNAs was done. We have earlier reported CPC's rare variants from whole exome sequencing across 18 affected samples in a total of 64 subjects. A Smith-Waterman algorithm was used to infer a couple of lncRNAs from WES samples of CPC with predictions from the Noncode database. Further screening and quantification using PCR, we ascertained interactions using Micro Scale Thermophoresis (MST). We report the role of lnc-EPB41-1-1 shown to be promiscuously interacting with KIF13A substantiating their role in regulation.

Subject Areas

long non coding RNA, whole exome sequencing, protein interaction, congenital pouch colon, microscale thermophoresis

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