Version 1
: Received: 30 August 2018 / Approved: 30 August 2018 / Online: 30 August 2018 (08:14:55 CEST)
How to cite:
Lee, H.; Seo, C.; Kim, Y.; Park, M.; Choi, B.; Ji, M.; Lee, S.; Paik, M. Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid. Preprints2018, 2018080519. https://doi.org/10.20944/preprints201808.0519.v1
Lee, H.; Seo, C.; Kim, Y.; Park, M.; Choi, B.; Ji, M.; Lee, S.; Paik, M. Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid. Preprints 2018, 2018080519. https://doi.org/10.20944/preprints201808.0519.v1
Lee, H.; Seo, C.; Kim, Y.; Park, M.; Choi, B.; Ji, M.; Lee, S.; Paik, M. Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid. Preprints2018, 2018080519. https://doi.org/10.20944/preprints201808.0519.v1
APA Style
Lee, H., Seo, C., Kim, Y., Park, M., Choi, B., Ji, M., Lee, S., & Paik, M. (2018). Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid. Preprints. https://doi.org/10.20944/preprints201808.0519.v1
Chicago/Turabian Style
Lee, H., Sooyeun Lee and Man-Jeong Paik. 2018 "Metabolomic Study of Polyamines in Rat Urine Following Intraperitoneal Injection of γ-Hydroxybutyric Acid" Preprints. https://doi.org/10.20944/preprints201808.0519.v1
Abstract
1) Background: Recently, illegal abuse of γ-hydroxybutyric acid (GHB) has increased in drug-facilitated crimes, but determination of GHB exposure and intoxication is difficult due to rapid metabolism of GHB. Its biochemical mechanism has not been completely investigated. And metabolomic study by polyamine profile and pattern analyses was not performed in rat urinefollowing intraperitoneal injection with GHB. 2) Methods: Polyamine profiling analysis by gas chromatography-mass spectrometry combined with star pattern recognition analysis was performed in this study. Multivariate statistical analysis was used to evaluate discrimination between control and GHB administration groups. 3) Results: Six polyamines were determined in control, single and multiple GHB administration groups. Star pattern showed distorted hexagonal shapes with characteristic and readily distinguishable patterns for each group. N1-Acetylspermine (p < 0.001), putrescine (p <0.006), N1-acetylspermidine (p <0.009), and spermine (p < 0.027) were significantly increased in single administration group but were significantly lower in the multiple administration group than in the control group. N1-Acetylspermine was the main polyamine for discrimination between control, single and multiple administration groups. Spermine showed similar levels in single and multiple administration groups. 4) Conclusions: The polyamine metabolic pattern was monitored in GHB administration groups. N1-Acetylspermine and spermine were evaluated as potential biomarkers of GHB exposure and addiction.
Keywords
metabolomics; γ-Hydroxybutyric acid; polyamine profiling analysis, gas chromatography-mass spectrometry; star pattern recognition analysis; multivariate analysis
Subject
Chemistry and Materials Science, Analytical Chemistry
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
