Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fabrication and Characterization of Electrospun PHA/Graphene Silver Nano-Composite Scaffold for Antibacterial Application

Abdul Mukheem
,
Kasturi Muthoosamy
,
Sivakumar Manickam
ORCID logo ,
Kumar Sudesh
,
Syed Shahabuddin
* ORCID logo ,
Rahman Saidur
,
Noor Akbar
,
Nanthini Sridewi
ORCID logo
Version 1 : Received: 14 August 2018 / Approved: 14 August 2018 / Online: 14 August 2018 (14:16:21 CEST)

A peer-reviewed article of this Preprint also exists.

Mukheem, A.; Muthoosamy, K.; Manickam, S.; Sudesh, K.; Shahabuddin, S.; Saidur, R.; Akbar, N.; Sridewi, N. Fabrication and Characterization of an Electrospun PHA/Graphene Silver Nanocomposite Scaffold for Antibacterial Applications. Materials 2018, 11, 1673. Mukheem, A.; Muthoosamy, K.; Manickam, S.; Sudesh, K.; Shahabuddin, S.; Saidur, R.; Akbar, N.; Sridewi, N. Fabrication and Characterization of an Electrospun PHA/Graphene Silver Nanocomposite Scaffold for Antibacterial Applications. Materials 2018, 11, 1673.

Abstract

Many wounds are unresponsive to current available treatment techniques and therefore there is an immense need to explore suitable materials including biomaterials, which are being considered as the crucial factor to accelerate the healing cascade. In this study, we fabricated polyhydroxyalkanoate based antibacterial mats via electrospun technique. One-pot green synthesized graphene decorated silver nanoparticles (GAg) were incorporated into the fibres of poly-3 hydroxybutarate-co-12 mol% hydroxyhexanoate (P3HB-co-12mol% HHx), a co-polymer of polyhydroxyalkanoate (PHA) family which is highly biocompatible, biodegradable and flexible in nature. The synthesised PHA/GAg biomaterial was characterized by field emission scanning electron microscopy (FESEM), elemental mapping, thermogravimetric analysis (TGA), UV-visible spectroscopy (UV-vis) and Fourier transform infrared spectroscopy (FTIR). In-vitro antibacterial analysis were performed to investigate the efficacy of PHA/GAg against gram positive Staphylococcus aureus (S.aureus) strain 12600 ATCC and gram negative Escherichia coli (E.coli) strain 8739 ATCC. The results indicated that the PHA/GAg demonstrated significant reduction of Staphylococcus aureus (S.aureus strain 12600 ATCC) and Escherichia coli (E.coli strain 8739 ATCC) as compared to bare PHA or PHA-rGO in 2 hours of time. The P value (P< 0.05) was obtained by using 2 sample T-test distribution.

Keywords

graphene; silver nanoparticles; PHA; electrospun biomaterial; antibacterial

Subject

Chemistry and Materials Science, Biomaterials

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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