Version 1
: Received: 6 August 2018 / Approved: 6 August 2018 / Online: 6 August 2018 (13:56:16 CEST)
How to cite:
Mirnajafizadeh, F.; Ramsey, D.; McAlpine, S.; Wang, F.; Reece, P.; Stride, J. Nanoparticles for Bioapplications: Study of the Cytotoxicity of Water Dispersible Quantum Dots. Preprints2018, 2018080123. https://doi.org/10.20944/preprints201808.0123.v1
Mirnajafizadeh, F.; Ramsey, D.; McAlpine, S.; Wang, F.; Reece, P.; Stride, J. Nanoparticles for Bioapplications: Study of the Cytotoxicity of Water Dispersible Quantum Dots. Preprints 2018, 2018080123. https://doi.org/10.20944/preprints201808.0123.v1
Mirnajafizadeh, F.; Ramsey, D.; McAlpine, S.; Wang, F.; Reece, P.; Stride, J. Nanoparticles for Bioapplications: Study of the Cytotoxicity of Water Dispersible Quantum Dots. Preprints2018, 2018080123. https://doi.org/10.20944/preprints201808.0123.v1
APA Style
Mirnajafizadeh, F., Ramsey, D., McAlpine, S., Wang, F., Reece, P., & Stride, J. (2018). Nanoparticles for Bioapplications: Study of the Cytotoxicity of Water Dispersible Quantum Dots. Preprints. https://doi.org/10.20944/preprints201808.0123.v1
Chicago/Turabian Style
Mirnajafizadeh, F., Peter Reece and John Stride. 2018 "Nanoparticles for Bioapplications: Study of the Cytotoxicity of Water Dispersible Quantum Dots" Preprints. https://doi.org/10.20944/preprints201808.0123.v1
Abstract
Semiconductor nanocrystals or quantum dots (QDs), have unique optical and physical properties that make them potential imaging tools in biological and medical applications. However, concerns such as the aqueous dispersivity, toxicity to cells and stability in biological environments may limit the use of QDs in bioapplications. Here, we report an investigation into the cytotoxicity of aqueously dispersed CdSe(S) and CdSe(S)/ZnO core/shell QDs in the presence of human colorectal carcinoma cells (HCT-116) and a human skin fibroblast cell line (WS-1). The cytotoxicity of the precursor solutions used in the synthesis of the CdSe(S) QDs was also determined in the presence of HCT-116 cells and compared to that of the heat-shock protein (Hsp90) inhibitor, 17-AAG. CdSe(S) QDs were found to have a low toxicity at concentrations up to 100 µg/ml, with a decreased cell viability at higher concentrations, indicating a highly dose-dependent response. Meanwhile, CdSe(S)/ZnO core/shell QDs exhibited lower toxicity than uncoated QDs at higher concentrations. Confocal microscopy images of HCT-116 cells after incubation with CdSe(S) and CdSe(S)/ZnO QDs showed that the cells were stable in aqueous concentrations of 100 µg of QDs per ml, with no sign of cell necrosis, confirming the cytotoxicity data. Key words: HCT-116, WS1, water dispersive QDs, aqueous synthesis, cytotoxicity of QDs.
Keywords
HCT-116, WS1, water dispersive QDs, aqueous synthesis, cytotoxicity of QDs.
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.