Preprint Article Version 1 This version is not peer-reviewed

Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in a AHL-Based E. coli Biosensor

Version 1 : Received: 20 July 2018 / Approved: 25 July 2018 / Online: 25 July 2018 (08:32:31 CEST)

A peer-reviewed article of this Preprint also exists.

Qin, X.; Emich, J.; Goycoolea, F.M. Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor. Biomolecules 2018, 8, 87. Qin, X.; Emich, J.; Goycoolea, F.M. Assessment of the Quorum Sensing Inhibition Activity of a Non-Toxic Chitosan in an N-Acyl Homoserine Lactone (AHL)-Based Escherichia coli Biosensor. Biomolecules 2018, 8, 87.

Journal reference: Biomolecules 2018, 8, 87
DOI: 10.3390/biom8030087

Abstract

New approaches to deal with drug-resistant pathogenic bacteria are urgent. We studied the antibacterial effect of chitosans against an E. coli quorum sensing biosensor reporter strain, and selected a non-toxic chitosan to evaluate its QS inhibition activity and its effect on bacterial aggregation. To this end, chitosans of varying DA (12 to 69%) and Mw (29 to 288 KDa) were studied. Only chitosans of low DA (~12%) inhibited the bacterial growth, regardless of the Mw. Chitosan MDP DA30 (DA 42% and Mw 115 kDa) was selected for further QS inhibition and SEM imaging studies. MDP DA30 chitosan exhibited QS inhibition activity in an inverse dose-dependent manner (≤12.5 µg/mL). SEM images revealed that this chitosan, when added at low concentration (≤30.6 µg/mL), induced substantial bacterial aggregation, whereas at high concentration (234.3 µg/mL), it did not. Aggregation explains the QS inhibition activity as the consequence of retardation of the diffusion of AHL.

Subject Areas

chitosan; quorum sensing; antibacterial activity; quorum sensing inhibition

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