Version 1
: Received: 4 July 2018 / Approved: 5 July 2018 / Online: 5 July 2018 (16:33:14 CEST)
How to cite:
Park, E.; Song, G.; Lee, S.; Kim, Y.; Park, K. Ex Vivo Rumex Crispus and Cordyceps Sinensis Mixture Regulates Immune Cells Responses to Pro-inflammatory Cytokines inC57BL/6 mice Splenocytes. Preprints2018, 2018070104. https://doi.org/10.20944/preprints201807.0104.v1
Park, E.; Song, G.; Lee, S.; Kim, Y.; Park, K. Ex Vivo Rumex Crispus and Cordyceps Sinensis Mixture Regulates Immune Cells Responses to Pro-inflammatory Cytokines inC57BL/6 mice Splenocytes. Preprints 2018, 2018070104. https://doi.org/10.20944/preprints201807.0104.v1
Park, E.; Song, G.; Lee, S.; Kim, Y.; Park, K. Ex Vivo Rumex Crispus and Cordyceps Sinensis Mixture Regulates Immune Cells Responses to Pro-inflammatory Cytokines inC57BL/6 mice Splenocytes. Preprints2018, 2018070104. https://doi.org/10.20944/preprints201807.0104.v1
APA Style
Park, E., Song, G., Lee, S., Kim, Y., & Park, K. (2018). <em>Ex Vivo Rumex Crispus</em> and <em>Cordyceps Sinensis</em> Mixture Regulates Immune Cells Responses to Pro-inflammatory Cytokines inC57BL/6 mice Splenocytes. Preprints. https://doi.org/10.20944/preprints201807.0104.v1
Chicago/Turabian Style
Park, E., Yong-Gyu Kim and Kun-Young Park. 2018 "<em>Ex Vivo Rumex Crispus</em> and <em>Cordyceps Sinensis</em> Mixture Regulates Immune Cells Responses to Pro-inflammatory Cytokines inC57BL/6 mice Splenocytes" Preprints. https://doi.org/10.20944/preprints201807.0104.v1
Abstract
We investigated the efficacy of a Rumex crispus and Cordyceps sinensis mixture made using the Beopje (Korea traditional processing method to remove anti-nutrients and enhance phytochemicals) method to regulate immune cell responses toward nitric oxide (NO) production, pro-inflammatory cytokines, and inflammation related genes in mice splenocytes. The six experimental groups were as follows: control (control), Rc-Cs (Rumex crispus (Rc) and Cordyceps sinensis (Cs) mixture, 6:4), TMC (Taemyeongcheong, commercial healthy drink containing Rc-Cs), LPS (lipopolysaccharide), LPS+Rc-Cs, and LPS+TMC. The Rc-Cs mixture reduced nitric oxide (NO) production in LPS-induced splenocytes. Moreover, Rc-Cs enhanced production of the pro-inflammatory cytokines TNF-α, IFN-γ, IL-1β, and IL-6 compared to the control (no treatment). However, Rc-Cs inhibited production of pro-inflammatory cytokines in LPS-induced splenocytes. In addition, LPS+Rc-Cs also significantly suppressed mRNA expression of IL-1β and IL-6 compared to LPS treatment. Interestingly, Rc-Cs did not increase mRNA levels of iNOS and COX-2, which are inflammation related genes compared to the control, while LPS+Rc-Cs reduced mRNA levels of iNOS and COX-2 compared LPS alone (p < 0.05). TMC showed a similar pattern compared to Rc-Cs. Therefore, Rc-Cs treatment in splenocytes enhanced NO production and pro-inflammatory cytokines compared to the control, whereas Rc-Cs treatment in LPS-induced splenocytes reduced NO production, pro-inflammatory cytokines, and inflammation related genes. Thus, Rc-Cs regulated immune cells responses by increasing pro-inflammatory cytokines in splenocytes and reducing toxin (LPS)-induced inflammation. These results indicate that a Rumex crispus and Cordyceps sinensis mixture (Rc-Cs) and TMC containing Rc-Cs promote immune cells responses and anti-inflammatory activities.
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
