Preprint Article Version 1 This version is not peer-reviewed

Single Cell Isolation Using Optical Tweezers

Version 1 : Received: 23 June 2018 / Approved: 25 June 2018 / Online: 25 June 2018 (06:10:34 CEST)

A peer-reviewed article of this Preprint also exists.

Keloth, A.; Anderson, O.; Risbridger, D.; Paterson, L. Single Cell Isolation Using Optical Tweezers. Micromachines 2018, 9, 434. Keloth, A.; Anderson, O.; Risbridger, D.; Paterson, L. Single Cell Isolation Using Optical Tweezers. Micromachines 2018, 9, 434.

Journal reference: Micromachines 2018, 9, 434
DOI: 10.3390/mi9090434

Abstract

Optical tweezers offer a non-contact method for selecting single cells and translocating them from one microenvironment to another. We have characterized the optical tweezing of yeast S. cerevisiae and can manipulate single cells at velocities up to 0.77 mm/s using laser powers of 40 mW from a 785 nm diode laser. We have fabricated and tested three cell isolation devices; a micropipette, a PDMS chip and laser machined fused silica chip and we have isolated single bacteria, yeast and cyanobacteria cells. The most effective isolation was achieved in PDMS chip, where single yeast cells were grown and observed for 18 hours without contamination. The duration of budding in S. cerevisiae was not affected by the laser parameters used, but the time from tweezing until the first budding event began increased with increase laser energy (laser power x time). Cells tweezed using 25 mW for 1 minute were viable after isolation. We have constructed a micro-consortium of yeast cells, and a co-culture of yeast and bacteria, using optical tweezers in combination with the PDMS network of channels and isolation chambers, which may impact on both industrial biotechnology and understanding pathogen dynamics.

Subject Areas

optical tweezers; optical trap; PDMS devices; single cells

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