Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated Solid-Phase Microextraction

Stefano Dugheri
* ORCID logo ,
Alessandro Bonari
,
Matteo Gentili
,
Giovanni Cappelli
,
Ilenia Pompilio
,
Costanza Bossi
,
Giulio Arcangeli
,
Marcello Campagna
,
Nicola Mucci
Version 1 : Received: 22 June 2018 / Approved: 22 June 2018 / Online: 22 June 2018 (16:05:55 CEST)

A peer-reviewed article of this Preprint also exists.

Dugheri, S.; Bonari, A.; Gentili, M.; Cappelli, G.; Pompilio, I.; Bossi, C.; Arcangeli, G.; Campagna, M.; Mucci, N. High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated Solid-Phase Microextraction. Molecules 2018, 23, 1869. Dugheri, S.; Bonari, A.; Gentili, M.; Cappelli, G.; Pompilio, I.; Bossi, C.; Arcangeli, G.; Campagna, M.; Mucci, N. High-Throughput Analysis of Selected Urinary Hydroxy Polycyclic Aromatic Hydrocarbons by an Innovative Automated Solid-Phase Microextraction. Molecules 2018, 23, 1869.

Abstract

High-throughput screening of samples is the strategy of choice to detect occupational exposure biomarkers, yet it requires user-friendly apparatus that gives relatively prompt results while ensuring high degrees of selectivity, precision, accuracy and automation, particularly in preparation processes. In the last 10 years, miniaturization has attracted much attention in analytical chemistry and has driven solvent and sample savings and easier automation, the latter thanks to the introduction on the market of three axis autosampler. In light of the above, this contribution describes a novel user-friendly solid-phase microextraction (SPME) off- and on-line platform coupled with gas chromatography triple quadrupole-mass spectrometry to determine urinary 1- and 2-hydroxy-napthalene, 9-hydroxy-phenantrene, 1-hydroxy-pyrene, 3- and 9-hydroxy-benzoantracene and 3-hydroxy-benzo[a]pyrene, metabolites of the related polycyclic aromatic hydrocarbons. In this new procedure chromatography’s sensitivity is combined with the user-friendliness of N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide on-fiber SPME derivatization previous direct immersion sampling, to which is added the quantitative accuracy afforded using specific isotope-labelled internal standards. The detection limits for the seven OH-PAHs were ranged from 0.28 to 1.87 ng/L. Intra-(from 2.5 to 3.0%) and inter-session (from 2.4 to 3.9%) repeatability was also evaluated. This method serves to identify suitable risk-control strategies for occupational hygiene conservation programs

Keywords

SPME; OH-PAHs; gas-chromatography; MTBSTFA.

Subject

Chemistry and Materials Science, Analytical Chemistry

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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