Preprint Article Version 1 This version is not peer-reviewed

Anti-melanogenic and Anti-oxidant Activities of an Ethanolic Extract of Kummerowia striata and its active compounds, p-coumaric acid and quercetin

Version 1 : Received: 21 June 2018 / Approved: 21 June 2018 / Online: 21 June 2018 (14:44:02 CEST)

How to cite: Park, J.; Lee, J.Y.; Cho, Y.; Ahn, E.; Jeong, W.; Shin, H.S.; Kim, M.; Oh, J.S. Anti-melanogenic and Anti-oxidant Activities of an Ethanolic Extract of Kummerowia striata and its active compounds, p-coumaric acid and quercetin. Preprints 2018, 2018060341 (doi: 10.20944/preprints201806.0341.v1). Park, J.; Lee, J.Y.; Cho, Y.; Ahn, E.; Jeong, W.; Shin, H.S.; Kim, M.; Oh, J.S. Anti-melanogenic and Anti-oxidant Activities of an Ethanolic Extract of Kummerowia striata and its active compounds, p-coumaric acid and quercetin. Preprints 2018, 2018060341 (doi: 10.20944/preprints201806.0341.v1).

Abstract

Kummerowia striata is a traditional medicine used for the therapy of inflammation-related diseases. Herein, we investigated the anti-melanogenic and antioxidant activities of an ethanolic extract of K. striata (EKS) using a number of in vitro and cell culture model systems. The anti-melanogenic effect was assessed in B16F10 melanoma cells based on melanin synthesis and in vitro tyrosinase inhibitory activity and the anti-oxidant activity assays were performed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2ʹ-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS); EKS exhibited strong anti-oxidant activities in both the assays. The expression of tyrosinase, tyrosinase-related protein 1, tyrosinase-related protein 2, and microphthalmia-associated transcription factor was decreased in a dose-dependent manner at mRNA and protein levels upon treatment with EKS. Notably, EKS did not affect the cell viability at all the EKS concentrations used in this study, indicating that EKS-mediated inhibition of melanin synthesis is not accompanied with cytotoxicity. Collectively, our findings demonstrate, for the first time, that EKS possesses anti-melanogenic and anti-oxidant activities, and suggest that further evaluation and development of EKS as a functional supplement or cosmetic might be useful for skin whitening and for reduction wrinkles.

Subject Areas

Kummerowia striata; anti-oxidant activity; melanin synthesis; p-coumaric acid; quercetin

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our diversity statement.

Leave a public comment
Send a private comment to the author(s)
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.