Preprint Article Version 2 This version is not peer-reviewed

Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation

Version 1 : Received: 20 June 2018 / Approved: 21 June 2018 / Online: 21 June 2018 (06:15:23 CEST)
Version 2 : Received: 26 June 2018 / Approved: 27 June 2018 / Online: 27 June 2018 (10:43:43 CEST)

How to cite: Lloris-Carsí, J.M.; Barrios-Pitarque, C.; Lloris-Cejalvo, J.M.; Gil-Santos, L.; Cejalvo-Lapeña, D. Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation. Preprints 2018, 2018060334 (doi: 10.20944/preprints201806.0334.v2). Lloris-Carsí, J.M.; Barrios-Pitarque, C.; Lloris-Cejalvo, J.M.; Gil-Santos, L.; Cejalvo-Lapeña, D. Tracking Healing Process of Experimental Liver Injuries Treated with Different Sealants and Adhesives Biomaterials. Matrix Metalloproteinase Evaluation. Preprints 2018, 2018060334 (doi: 10.20944/preprints201806.0334.v2).

Abstract

. Sealants and adhesives are used in the repair and preservation of damaged solid organs. This study examines the matrix metalloproteinases (MMP) activity in the healing of liver injuries treated with two biological adhesives (Tachosil® and GelitaSpon®) as well as that of a new elastic cyanoacrylate (Adhflex®). Methods. We induced in 90 male rats hepatic lesions using a Stiefel biopsy punch in the liver. Wound healing was assessed 2, 6, and 18 days after injury by quantifying MMP1, 2, 8, 9, and 13 tissue levels. The histopathological repair was evaluated by hematoxylin-eosin, Masson’s trichrome, and Periodic Acid Schiff (PAS) staining and CD31, CD68 immunohistochemical marker. The three sealants used contributed to the complete healing of hepatic lesions. Both histopathology and MMP findings point to the fact that degradation with Adhflex® is slower and causes a strong inflammatory reaction at the onset of healing. Results. All the MMPs measured showed higher values early in the healing process in animals treated with Adhflex® and Tachosil, expression for MMP2 and MMP9 being significantly higher in the Adhflex-treated group. Animals treated with Tachosil had significant greater values of MMP8 and MPP13 than the Adhflex group. Animals treated with Adhflex® showed a sustained overexpression in all MMPs even at the latest wound healing stages. Conclusion. Notably, the overexpression of the MMPs did not negatively influence the histological healing process of liver injuries. Since all hepatic trauma injuries should be treated as emergencies, any easy-to-use and rapid sealant, like Adhflex®, could be considered an adequate treatment option.

Subject Areas

hepatic injury; sealants; metalloproteinases; inflammatory response; wound healing

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