Preprint Article Version 1 This version is not peer-reviewed

Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue

Version 1 : Received: 16 June 2018 / Approved: 19 June 2018 / Online: 19 June 2018 (15:28:53 CEST)

A peer-reviewed article of this Preprint also exists.

Kobayashi, T.; Nakata, K.; Yajima, I.; Kato, M.; Tsurui, H. Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue. Bioengineering 2018, 5, 67. Kobayashi, T.; Nakata, K.; Yajima, I.; Kato, M.; Tsurui, H. Label-Free Imaging of Melanoma with Confocal Photothermal Microscopy: Differentiation between Malignant and Benign Tissue. Bioengineering 2018, 5, 67.

Journal reference: Bioengineering 2018, 5, 67
DOI: 10.3390/bioengineering5030067

Abstract

Label-free confocal photothermal (CPT) microscopy was utilized for the first time to investigate malignancy in mouse skin cells. A laser diode (LD) with 405nm or 488nm was used as a pump and 638nm LD as a probe for the CPT microscope. The Grey Level Cooccurrence Matrix (GLCM) for texture analysis was applied to the CPT images. Nine parameters of GLCM were calculated for the intracellular super-resolved CPT images, and the parameters Entropy and Prominence were found to be most suited among the nine parameters to discriminate between healthy cells and MM cells in case pump wavelength of 488nm is used.

Subject Areas

photothermal microscopy; label-free imaging; melanoma; texture analysis

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