Preprint Article Version 1 This version is not peer-reviewed

Z Probe, an Efficient Tool for Characterizing Long Non-Coding RNA in FFPE Tissues

Version 1 : Received: 6 June 2018 / Approved: 6 June 2018 / Online: 6 June 2018 (13:05:30 CEST)

How to cite: Tripathi, M.; Zacheaus, C.; Doxtater, K.; Keramatnia, F.; Gao, L.; Jaggi, M.; Yallapu, M.; Chauhan, S. Z Probe, an Efficient Tool for Characterizing Long Non-Coding RNA in FFPE Tissues. Preprints 2018, 2018060096 (doi: 10.20944/preprints201806.0096.v1). Tripathi, M.; Zacheaus, C.; Doxtater, K.; Keramatnia, F.; Gao, L.; Jaggi, M.; Yallapu, M.; Chauhan, S. Z Probe, an Efficient Tool for Characterizing Long Non-Coding RNA in FFPE Tissues. Preprints 2018, 2018060096 (doi: 10.20944/preprints201806.0096.v1).

Abstract

Formalin-Fixed Paraffin Embedded (FFPE) tissues are a valuable resource in studying different markers and mechanistic molecules (protein, DNA and RNA) in order to understand the etiology of different cancers as well as many other diseases. Degradation and modification of RNA is the major challenge in utilizing FFPE tissue samples in medical research. Recently, non-protein coding transcripts long non-coding RNAs (lncRNAs), have gained significant attention due to their important biological actions and potential involvement in cancer. There is no validated method except qRTPCR or RNAseq to evaluate and study lncRNA expression. We have standardized and are reporting a sensitive Z probe based in situ hybridization method to identify, localize and quantitate lncRNA in FFPE tissues. This assay is sensitive to single transcript and localizes lncRNA in individual cells within tumor. We have characterized a tumor suppressor lncRNA-NRON (non coding repressor of NFAT), which is scarcely expressed, a moderately expressed oncogeneic lncRNA UCA1 (urothelial cancer associated 1), and a highly studied and expressed lncRNA MALAT1 (metastasis associated lung adenocarcinoma transcript1) in different cancers. High MALAT1 staining was found in colorectal, breast and pancreatic cancer. MALAT1 expression increased with the progression of the stage in colorectal cancer and invasiveness in breast cancer.

Subject Areas

long noncoding RNA; MALAT1; UCA1; NRON; Z probe; colorectal cancer; pancreatic cancer; breast cancer

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